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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Draba nemorosa Protein psbN (psbN), partial CSB-YP392482DIE
CSB-EP392482DIE
CSB-BP392482DIE
CSB-MP392482DIE
CSB-EP392482DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP392483DIE
CSB-EP392483DIE
CSB-BP392483DIE
CSB-MP392483DIE
CSB-EP392483DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP392484DIE
CSB-EP392484DIE
CSB-BP392484DIE
CSB-MP392484DIE
CSB-EP392484DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Putative uncharacterized protein ycf15 (ycf15-A) CSB-YP392485DIE
CSB-EP392485DIE
CSB-BP392485DIE
CSB-MP392485DIE
CSB-EP392485DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP392486DIE
CSB-EP392486DIE
CSB-BP392486DIE
CSB-MP392486DIE
CSB-EP392486DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP392487DIE
CSB-EP392487DIE
CSB-BP392487DIE
CSB-MP392487DIE
CSB-EP392487DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP392488DIE1
CSB-EP392488DIE1
CSB-BP392488DIE1
CSB-MP392488DIE1
CSB-EP392488DIE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa NAD (P)H-quinone oxidoreductase subunit 1, chloroplastic, partial CSB-YP392489DIE
CSB-EP392489DIE
CSB-BP392489DIE
CSB-MP392489DIE
CSB-EP392489DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Putative membrane protein ycf1 (ycf1-A), partial CSB-YP392490DIE
CSB-EP392490DIE
CSB-BP392490DIE
CSB-MP392490DIE
CSB-EP392490DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum Photosystem II reaction center protein I (psbI), partial CSB-YP392491LLP
CSB-EP392491LLP
CSB-BP392491LLP
CSB-MP392491LLP
CSB-EP392491LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP392492LLP1
CSB-EP392492LLP1
CSB-BP392492LLP1
CSB-MP392492LLP1
CSB-EP392492LLP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP392493LLP
CSB-EP392493LLP
CSB-BP392493LLP
CSB-MP392493LLP
CSB-EP392493LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum Photosystem II reaction center protein M (psbM), partial CSB-YP392494LLP
CSB-EP392494LLP
CSB-BP392494LLP
CSB-MP392494LLP
CSB-EP392494LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP392495LLP
CSB-EP392495LLP
CSB-BP392495LLP
CSB-MP392495LLP
CSB-EP392495LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP392496LLP
CSB-EP392496LLP
CSB-BP392496LLP
CSB-MP392496LLP
CSB-EP392496LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum ATP synthase subunit beta, chloroplastic (atpB), partial CSB-YP392497LLP
CSB-EP392497LLP
CSB-BP392497LLP
CSB-MP392497LLP
CSB-EP392497LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP392498LLP1
CSB-EP392498LLP1
CSB-BP392498LLP1
CSB-MP392498LLP1
CSB-EP392498LLP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum Photosystem II reaction center protein L (psbL), partial CSB-YP392499LLP
CSB-EP392499LLP
CSB-BP392499LLP
CSB-MP392499LLP
CSB-EP392499LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP392500LLP
CSB-EP392500LLP
CSB-BP392500LLP
CSB-MP392500LLP
CSB-EP392500LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP392501LLP1
CSB-EP392501LLP1
CSB-BP392501LLP1
CSB-MP392501LLP1
CSB-EP392501LLP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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