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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lepidium virginicum 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP392502LLP
CSB-EP392502LLP
CSB-BP392502LLP
CSB-MP392502LLP
CSB-EP392502LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP392503LLP
CSB-EP392503LLP
CSB-BP392503LLP
CSB-MP392503LLP
CSB-EP392503LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP392504LLP
CSB-EP392504LLP
CSB-BP392504LLP
CSB-MP392504LLP
CSB-EP392504LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum Putative uncharacterized protein ycf15 (ycf15-A) CSB-YP392505LLP
CSB-EP392505LLP
CSB-BP392505LLP
CSB-MP392505LLP
CSB-EP392505LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic, partial CSB-YP392506LLP
CSB-EP392506LLP
CSB-BP392506LLP
CSB-MP392506LLP
CSB-EP392506LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP392507LLP
CSB-EP392507LLP
CSB-BP392507LLP
CSB-MP392507LLP
CSB-EP392507LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum NAD (P)H-quinone oxidoreductase subunit I, chloroplastic CSB-YP392508LLP
CSB-EP392508LLP
CSB-BP392508LLP
CSB-MP392508LLP
CSB-EP392508LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidium virginicum 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP392509LLP
CSB-EP392509LLP
CSB-BP392509LLP
CSB-MP392509LLP
CSB-EP392509LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP392510LLU1
CSB-EP392510LLU1
CSB-BP392510LLU1
CSB-MP392510LLU1
CSB-EP392510LLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP392511LLU
CSB-EP392511LLU
CSB-BP392511LLU
CSB-MP392511LLU
CSB-EP392511LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP392512LLU
CSB-EP392512LLU
CSB-BP392512LLU
CSB-MP392512LLU
CSB-EP392512LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima ATP synthase subunit beta, chloroplastic (atpB) CSB-YP392513LLU
CSB-EP392513LLU
CSB-BP392513LLU
CSB-MP392513LLU
CSB-EP392513LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Photosystem II reaction center protein J (psbJ), partial CSB-YP392514LLU
CSB-EP392514LLU
CSB-BP392514LLU
CSB-MP392514LLU
CSB-EP392514LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP392515LLU
CSB-EP392515LLU
CSB-BP392515LLU
CSB-MP392515LLU
CSB-EP392515LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP392516LLU
CSB-EP392516LLU
CSB-BP392516LLU
CSB-MP392516LLU
CSB-EP392516LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP392517LLU
CSB-EP392517LLU
CSB-BP392517LLU
CSB-MP392517LLU
CSB-EP392517LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP392518LLU
CSB-EP392518LLU
CSB-BP392518LLU
CSB-MP392518LLU
CSB-EP392518LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP392519LLU
CSB-EP392519LLU
CSB-BP392519LLU
CSB-MP392519LLU
CSB-EP392519LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima NAD (P)H-quinone oxidoreductase chain 4, chloroplastic, partial CSB-YP392520LLU
CSB-EP392520LLU
CSB-BP392520LLU
CSB-MP392520LLU
CSB-EP392520LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima NAD (P)H-quinone oxidoreductase subunit I, chloroplastic CSB-YP392521LLU
CSB-EP392521LLU
CSB-BP392521LLU
CSB-MP392521LLU
CSB-EP392521LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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