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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium kluyveri Energy-coupling factor transporter transmembrane protein EcfT (ecfT), partial CSB-YP402848CWX1
CSB-EP402848CWX1
CSB-BP402848CWX1
CSB-MP402848CWX1
CSB-EP402848CWX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 7-cyano-7-deazaguanine synthase (queC) CSB-YP402849CWX
CSB-EP402849CWX
CSB-BP402849CWX
CSB-MP402849CWX
CSB-EP402849CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP402850CWX
CSB-EP402850CWX
CSB-BP402850CWX
CSB-MP402850CWX
CSB-EP402850CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP402851CWX
CSB-EP402851CWX
CSB-BP402851CWX
CSB-MP402851CWX
CSB-EP402851CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Homoserine kinase (thrB) CSB-YP402852CWX
CSB-EP402852CWX
CSB-BP402852CWX
CSB-MP402852CWX
CSB-EP402852CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Cell division topological specificity factor (minE) CSB-YP402853CWX
CSB-EP402853CWX
CSB-BP402853CWX
CSB-MP402853CWX
CSB-EP402853CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Elongation factor 4 (lepA), partial CSB-YP402854CWX
CSB-EP402854CWX
CSB-BP402854CWX
CSB-MP402854CWX
CSB-EP402854CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP402855CWX
CSB-EP402855CWX
CSB-BP402855CWX
CSB-MP402855CWX
CSB-EP402855CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP402856CWX
CSB-EP402856CWX
CSB-BP402856CWX
CSB-MP402856CWX
CSB-EP402856CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Arginine repressor (argR) CSB-YP402857CWX
CSB-EP402857CWX
CSB-BP402857CWX
CSB-MP402857CWX
CSB-EP402857CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Histidinol-phosphate aminotransferase (hisC) CSB-YP402858CWX
CSB-EP402858CWX
CSB-BP402858CWX
CSB-MP402858CWX
CSB-EP402858CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri UPF0473 protein CKL_1327 (CKL_1327) CSB-YP402859CWX
CSB-EP402859CWX
CSB-BP402859CWX
CSB-MP402859CWX
CSB-EP402859CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP402860CWX
CSB-EP402860CWX
CSB-BP402860CWX
CSB-MP402860CWX
CSB-EP402860CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Ribosome maturation factor RimM (rimM) CSB-YP402861CWX
CSB-EP402861CWX
CSB-BP402861CWX
CSB-MP402861CWX
CSB-EP402861CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri UPF0102 protein CKL_1410 (CKL_1410) CSB-YP402862CWX
CSB-EP402862CWX
CSB-BP402862CWX
CSB-MP402862CWX
CSB-EP402862CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Ribosome-binding factor A (rbfA) CSB-YP402863CWX
CSB-EP402863CWX
CSB-BP402863CWX
CSB-MP402863CWX
CSB-EP402863CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Potassium-transporting ATPase C chain (kdpC), partial CSB-YP402864CWX1
CSB-EP402864CWX1
CSB-BP402864CWX1
CSB-MP402864CWX1
CSB-EP402864CWX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri LexA repressor (lexA) CSB-YP402865CWX
CSB-EP402865CWX
CSB-BP402865CWX
CSB-MP402865CWX
CSB-EP402865CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine UPF0480 protein C15orf24 homolog, partial CSB-YP402866BO
CSB-EP402866BO
CSB-BP402866BO
CSB-MP402866BO
CSB-EP402866BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Prothymosin alpha-B (ptmab) CSB-YP402867DIL
CSB-EP402867DIL
CSB-BP402867DIL
CSB-MP402867DIL
CSB-EP402867DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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