Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium kluyveri Octanoyltransferase (lipB) CSB-YP402828CWX
CSB-EP402828CWX
CSB-BP402828CWX
CSB-MP402828CWX
CSB-EP402828CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP402829CWX
CSB-EP402829CWX
CSB-BP402829CWX
CSB-MP402829CWX
CSB-EP402829CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri D-alanine--D-alanine ligase (ddl) CSB-YP402830CWX
CSB-EP402830CWX
CSB-BP402830CWX
CSB-MP402830CWX
CSB-EP402830CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L20 (rplT) CSB-YP402831CWX
CSB-EP402831CWX
CSB-BP402831CWX
CSB-MP402831CWX
CSB-EP402831CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Trigger factor (tig) CSB-YP402832CWX
CSB-EP402832CWX
CSB-BP402832CWX
CSB-MP402832CWX
CSB-EP402832CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Phosphoglycerate kinase (pgk) CSB-YP402833CWX
CSB-EP402833CWX
CSB-BP402833CWX
CSB-MP402833CWX
CSB-EP402833CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri UPF0042 nucleotide-binding protein CKL_3564 (CKL_3564) CSB-YP402834CWX
CSB-EP402834CWX
CSB-BP402834CWX
CSB-MP402834CWX
CSB-EP402834CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri ATP synthase epsilon chain (atpC) CSB-YP402835CWX
CSB-EP402835CWX
CSB-BP402835CWX
CSB-MP402835CWX
CSB-EP402835CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L31 (rpmE) CSB-YP402836CWX
CSB-EP402836CWX
CSB-BP402836CWX
CSB-MP402836CWX
CSB-EP402836CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Sugar fermentation stimulation protein homolog (sfsA) CSB-YP402837CWX
CSB-EP402837CWX
CSB-BP402837CWX
CSB-MP402837CWX
CSB-EP402837CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP402838CWX
CSB-EP402838CWX
CSB-BP402838CWX
CSB-MP402838CWX
CSB-EP402838CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L34 (rpmH) CSB-YP402839CWX
CSB-EP402839CWX
CSB-BP402839CWX
CSB-MP402839CWX
CSB-EP402839CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP402840CWX
CSB-EP402840CWX
CSB-BP402840CWX
CSB-MP402840CWX
CSB-EP402840CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Type III pantothenate kinase (coaX) CSB-YP402841CWX
CSB-EP402841CWX
CSB-BP402841CWX
CSB-MP402841CWX
CSB-EP402841CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP402842CWX
CSB-EP402842CWX
CSB-BP402842CWX
CSB-MP402842CWX
CSB-EP402842CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP402843CWX
CSB-EP402843CWX
CSB-BP402843CWX
CSB-MP402843CWX
CSB-EP402843CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L3 (rplC) CSB-YP402844CWX
CSB-EP402844CWX
CSB-BP402844CWX
CSB-MP402844CWX
CSB-EP402844CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L16 (rplP) CSB-YP402845CWX
CSB-EP402845CWX
CSB-BP402845CWX
CSB-MP402845CWX
CSB-EP402845CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L24 (rplX) CSB-YP402846CWX
CSB-EP402846CWX
CSB-BP402846CWX
CSB-MP402846CWX
CSB-EP402846CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S13 (rpsM) CSB-YP402847CWX
CSB-EP402847CWX
CSB-BP402847CWX
CSB-MP402847CWX
CSB-EP402847CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>