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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella ovis 50S ribosomal protein L15 (rplO) CSB-YP403128BPI
CSB-EP403128BPI
CSB-BP403128BPI
CSB-MP403128BPI
CSB-EP403128BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S8 (rpsH) CSB-YP403129BPI
CSB-EP403129BPI
CSB-BP403129BPI
CSB-MP403129BPI
CSB-EP403129BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L4 (rplD) CSB-YP403130BPI
CSB-EP403130BPI
CSB-BP403130BPI
CSB-MP403130BPI
CSB-EP403130BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S12 (rpsL) CSB-YP403131BPI
CSB-EP403131BPI
CSB-BP403131BPI
CSB-MP403131BPI
CSB-EP403131BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP403132BPI
CSB-EP403132BPI
CSB-BP403132BPI
CSB-MP403132BPI
CSB-EP403132BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP403133BPI
CSB-EP403133BPI
CSB-BP403133BPI
CSB-MP403133BPI
CSB-EP403133BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP403134BPI
CSB-EP403134BPI
CSB-BP403134BPI
CSB-MP403134BPI
CSB-EP403134BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Peptidyl-tRNA hydrolase (pth) CSB-YP403135BPI
CSB-EP403135BPI
CSB-BP403135BPI
CSB-MP403135BPI
CSB-EP403135BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L32 (rpmF) CSB-YP403136BPI
CSB-EP403136BPI
CSB-BP403136BPI
CSB-MP403136BPI
CSB-EP403136BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP403137BPI
CSB-EP403137BPI
CSB-BP403137BPI
CSB-MP403137BPI
CSB-EP403137BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis tRNA (guanine-N (1)-)-methyltransferase CSB-YP403138BPI
CSB-EP403138BPI
CSB-BP403138BPI
CSB-MP403138BPI
CSB-EP403138BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Diaminopimelate epimerase (dapF) CSB-YP403139BPI
CSB-EP403139BPI
CSB-BP403139BPI
CSB-MP403139BPI
CSB-EP403139BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 3-dehydroquinate synthase (aroB) CSB-YP403140BPI
CSB-EP403140BPI
CSB-BP403140BPI
CSB-MP403140BPI
CSB-EP403140BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Protein-export protein secB (secB) CSB-YP403141BPI
CSB-EP403141BPI
CSB-BP403141BPI
CSB-MP403141BPI
CSB-EP403141BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP403142BPI
CSB-EP403142BPI
CSB-BP403142BPI
CSB-MP403142BPI
CSB-EP403142BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Ribosome maturation factor RimP (rimP) CSB-YP403143BPI
CSB-EP403143BPI
CSB-BP403143BPI
CSB-MP403143BPI
CSB-EP403143BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis ATP phosphoribosyltransferase (hisG) CSB-YP403144BPI
CSB-EP403144BPI
CSB-BP403144BPI
CSB-MP403144BPI
CSB-EP403144BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Chaperone-like protein hdeA (hdeA) CSB-YP403145BPI
CSB-EP403145BPI
CSB-BP403145BPI
CSB-MP403145BPI
CSB-EP403145BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis N-acetylneuraminate epimerase (nanM) CSB-YP403146BPI
CSB-EP403146BPI
CSB-BP403146BPI
CSB-MP403146BPI
CSB-EP403146BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP403147MON
CSB-EP403147MON
CSB-BP403147MON
CSB-MP403147MON
CSB-EP403147MON-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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