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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sphingomonas wittichii 30S ribosomal protein S19 (rpsS) CSB-YP403068SUF
CSB-EP403068SUF
CSB-BP403068SUF
CSB-MP403068SUF
CSB-EP403068SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S7 (rpsG) CSB-YP403069SUF
CSB-EP403069SUF
CSB-BP403069SUF
CSB-MP403069SUF
CSB-EP403069SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Acetaldehyde dehydrogenase 1/2 (Swit_1640) CSB-YP403070SUF
CSB-EP403070SUF
CSB-BP403070SUF
CSB-MP403070SUF
CSB-EP403070SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Protein TolB (tolB) CSB-YP403071SUF
CSB-EP403071SUF
CSB-BP403071SUF
CSB-MP403071SUF
CSB-EP403071SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii UPF0391 membrane protein Swit_2334 (Swit_2334), partial CSB-YP403072SUF1
CSB-EP403072SUF1
CSB-BP403072SUF1
CSB-MP403072SUF1
CSB-EP403072SUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Putative arginyl-tRNA--protein transferase (ate) CSB-YP403073SUF
CSB-EP403073SUF
CSB-BP403073SUF
CSB-MP403073SUF
CSB-EP403073SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP403074SUF
CSB-EP403074SUF
CSB-BP403074SUF
CSB-MP403074SUF
CSB-EP403074SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L33 (rpmG) CSB-YP403075SUF
CSB-EP403075SUF
CSB-BP403075SUF
CSB-MP403075SUF
CSB-EP403075SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP403076SUF
CSB-EP403076SUF
CSB-BP403076SUF
CSB-MP403076SUF
CSB-EP403076SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP403077SUF
CSB-EP403077SUF
CSB-BP403077SUF
CSB-MP403077SUF
CSB-EP403077SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii LexA repressor (lexA) CSB-YP403078SUF
CSB-EP403078SUF
CSB-BP403078SUF
CSB-MP403078SUF
CSB-EP403078SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP403079SUF
CSB-EP403079SUF
CSB-BP403079SUF
CSB-MP403079SUF
CSB-EP403079SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP403080SUF
CSB-EP403080SUF
CSB-BP403080SUF
CSB-MP403080SUF
CSB-EP403080SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP403081SUF
CSB-EP403081SUF
CSB-BP403081SUF
CSB-MP403081SUF
CSB-EP403081SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii D-alanine--D-alanine ligase (ddl) CSB-YP403082SUF
CSB-EP403082SUF
CSB-BP403082SUF
CSB-MP403082SUF
CSB-EP403082SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP403083SUF
CSB-EP403083SUF
CSB-BP403083SUF
CSB-MP403083SUF
CSB-EP403083SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Glycerol kinase (glpK), partial CSB-YP403084SUF
CSB-EP403084SUF
CSB-BP403084SUF
CSB-MP403084SUF
CSB-EP403084SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Adenylosuccinate synthetase (purA) CSB-YP403085SUF
CSB-EP403085SUF
CSB-BP403085SUF
CSB-MP403085SUF
CSB-EP403085SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Chorismate synthase (aroC) CSB-YP403086SUF
CSB-EP403086SUF
CSB-BP403086SUF
CSB-MP403086SUF
CSB-EP403086SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S20 (rpsT) CSB-YP403087SUF
CSB-EP403087SUF
CSB-BP403087SUF
CSB-MP403087SUF
CSB-EP403087SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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