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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanobrevibacter smithii 30S ribosomal protein S13P (rps13p) CSB-YP403308MNN
CSB-EP403308MNN
CSB-BP403308MNN
CSB-MP403308MNN
CSB-EP403308MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Chorismate synthase (aroC) CSB-YP403309MNN
CSB-EP403309MNN
CSB-BP403309MNN
CSB-MP403309MNN
CSB-EP403309MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Ribose-phosphate pyrophosphokinase (prs) CSB-YP403310MNN
CSB-EP403310MNN
CSB-BP403310MNN
CSB-MP403310MNN
CSB-EP403310MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Putative pterin-4-alpha-carbinolamine dehydratase (RoseRS_0192) CSB-YP403311RIQ
CSB-EP403311RIQ
CSB-BP403311RIQ
CSB-MP403311RIQ
CSB-EP403311RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP403312RIQ
CSB-EP403312RIQ
CSB-BP403312RIQ
CSB-MP403312RIQ
CSB-EP403312RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S18 1 (rpsR1) CSB-YP403313RIQ
CSB-EP403313RIQ
CSB-BP403313RIQ
CSB-MP403313RIQ
CSB-EP403313RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. UPF0758 protein RoseRS_0767 (RoseRS_0767) CSB-YP403314RIQ
CSB-EP403314RIQ
CSB-BP403314RIQ
CSB-MP403314RIQ
CSB-EP403314RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP403315RIQ
CSB-EP403315RIQ
CSB-BP403315RIQ
CSB-MP403315RIQ
CSB-EP403315RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP403316RIQ
CSB-EP403316RIQ
CSB-BP403316RIQ
CSB-MP403316RIQ
CSB-EP403316RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Translation initiation factor IF-1 (infA) CSB-YP403317RIQ
CSB-EP403317RIQ
CSB-BP403317RIQ
CSB-MP403317RIQ
CSB-EP403317RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S14 type Z (rpsZ) CSB-YP403318RIQ
CSB-EP403318RIQ
CSB-BP403318RIQ
CSB-MP403318RIQ
CSB-EP403318RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Elongation factor Tu 1 (tuf1) CSB-YP403319RIQ
CSB-EP403319RIQ
CSB-BP403319RIQ
CSB-MP403319RIQ
CSB-EP403319RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S16 (rpsP) CSB-YP403320RIQ
CSB-EP403320RIQ
CSB-BP403320RIQ
CSB-MP403320RIQ
CSB-EP403320RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Phosphoglycerate kinase (pgk) CSB-YP403321RIQ
CSB-EP403321RIQ
CSB-BP403321RIQ
CSB-MP403321RIQ
CSB-EP403321RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. UPF0102 protein RoseRS_1723 (RoseRS_1723) CSB-YP403322RIQ
CSB-EP403322RIQ
CSB-BP403322RIQ
CSB-MP403322RIQ
CSB-EP403322RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Glycerol kinase (glpK) CSB-YP403323RIQ
CSB-EP403323RIQ
CSB-BP403323RIQ
CSB-MP403323RIQ
CSB-EP403323RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Aspartate--tRNA ligase (aspS), partial CSB-YP403324RIQ
CSB-EP403324RIQ
CSB-BP403324RIQ
CSB-MP403324RIQ
CSB-EP403324RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP403325RIQ
CSB-EP403325RIQ
CSB-BP403325RIQ
CSB-MP403325RIQ
CSB-EP403325RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. D-tyrosyl-tRNA (Tyr) deacylase CSB-YP403326RIQ
CSB-EP403326RIQ
CSB-BP403326RIQ
CSB-MP403326RIQ
CSB-EP403326RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Phosphopantetheine adenylyltransferase (coaD) CSB-YP403327RIQ
CSB-EP403327RIQ
CSB-BP403327RIQ
CSB-MP403327RIQ
CSB-EP403327RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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