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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sphingomonas wittichii Proline--tRNA ligase (proS) CSB-YP403348SUF
CSB-EP403348SUF
CSB-BP403348SUF
CSB-MP403348SUF
CSB-EP403348SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP403349SUF
CSB-EP403349SUF
CSB-BP403349SUF
CSB-MP403349SUF
CSB-EP403349SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L1 (rplA) CSB-YP403350SUF
CSB-EP403350SUF
CSB-BP403350SUF
CSB-MP403350SUF
CSB-EP403350SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii tRNA (guanine-N (1)-)-methyltransferase CSB-YP403351SUF
CSB-EP403351SUF
CSB-BP403351SUF
CSB-MP403351SUF
CSB-EP403351SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP403352SUF
CSB-EP403352SUF
CSB-BP403352SUF
CSB-MP403352SUF
CSB-EP403352SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii ATP phosphoribosyltransferase (hisG) CSB-YP403353SUF
CSB-EP403353SUF
CSB-BP403353SUF
CSB-MP403353SUF
CSB-EP403353SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii UPF0260 protein Swit_2819 (Swit_2819) CSB-YP403354SUF
CSB-EP403354SUF
CSB-BP403354SUF
CSB-MP403354SUF
CSB-EP403354SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Acetylglutamate kinase (argB) CSB-YP403355SUF
CSB-EP403355SUF
CSB-BP403355SUF
CSB-MP403355SUF
CSB-EP403355SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Elongation factor P (efp) CSB-YP403356SUF
CSB-EP403356SUF
CSB-BP403356SUF
CSB-MP403356SUF
CSB-EP403356SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Indole-3-glycerol phosphate synthase (trpC) CSB-YP403357SUF
CSB-EP403357SUF
CSB-BP403357SUF
CSB-MP403357SUF
CSB-EP403357SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP403358SUF
CSB-EP403358SUF
CSB-BP403358SUF
CSB-MP403358SUF
CSB-EP403358SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Orotate phosphoribosyltransferase (pyrE) CSB-YP403359SUF
CSB-EP403359SUF
CSB-BP403359SUF
CSB-MP403359SUF
CSB-EP403359SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP403360SUF
CSB-EP403360SUF
CSB-BP403360SUF
CSB-MP403360SUF
CSB-EP403360SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii DNA repair protein recO (recO) CSB-YP403361SUF
CSB-EP403361SUF
CSB-BP403361SUF
CSB-MP403361SUF
CSB-EP403361SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Heme A synthase (ctaA), partial CSB-YP403362SUF
CSB-EP403362SUF
CSB-BP403362SUF
CSB-MP403362SUF
CSB-EP403362SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP403363SUF
CSB-EP403363SUF
CSB-BP403363SUF
CSB-MP403363SUF
CSB-EP403363SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Chromosomal replication initiator protein DnaA (dnaA) CSB-YP403364LLF
CSB-EP403364LLF
CSB-BP403364LLF
CSB-MP403364LLF
CSB-EP403364LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP403365LLF
CSB-EP403365LLF
CSB-BP403365LLF
CSB-MP403365LLF
CSB-EP403365LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP403366LLF
CSB-EP403366LLF
CSB-BP403366LLF
CSB-MP403366LLF
CSB-EP403366LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP403367LLF
CSB-EP403367LLF
CSB-BP403367LLF
CSB-MP403367LLF
CSB-EP403367LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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