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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sphingomonas wittichii 10 kDa chaperonin (groS) CSB-YP404170SUF
CSB-EP404170SUF
CSB-BP404170SUF
CSB-MP404170SUF
CSB-EP404170SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L10 (rplJ) CSB-YP404171SUF
CSB-EP404171SUF
CSB-BP404171SUF
CSB-MP404171SUF
CSB-EP404171SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Integration host factor subunit alpha (ihfA) CSB-YP404172SUF
CSB-EP404172SUF
CSB-BP404172SUF
CSB-MP404172SUF
CSB-EP404172SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S15 (rpsO) CSB-YP404173SUF
CSB-EP404173SUF
CSB-BP404173SUF
CSB-MP404173SUF
CSB-EP404173SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP404174SUF
CSB-EP404174SUF
CSB-BP404174SUF
CSB-MP404174SUF
CSB-EP404174SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Imidazolonepropionase (hutI) CSB-YP404175SUF
CSB-EP404175SUF
CSB-BP404175SUF
CSB-MP404175SUF
CSB-EP404175SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP404176SUF
CSB-EP404176SUF
CSB-BP404176SUF
CSB-MP404176SUF
CSB-EP404176SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 4-hydroxy-2-oxovalerate aldolase 3 (Swit_4923) CSB-YP404177SUF
CSB-EP404177SUF
CSB-BP404177SUF
CSB-MP404177SUF
CSB-EP404177SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Adenylosuccinate synthetase (purA) CSB-YP404178LLF
CSB-EP404178LLF
CSB-BP404178LLF
CSB-MP404178LLF
CSB-EP404178LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP404179LLF
CSB-EP404179LLF
CSB-BP404179LLF
CSB-MP404179LLF
CSB-EP404179LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Tyrosine--tRNA ligase (tyrS) CSB-YP404180LLF
CSB-EP404180LLF
CSB-BP404180LLF
CSB-MP404180LLF
CSB-EP404180LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L31 type B (rpmE2) CSB-YP404181LLF
CSB-EP404181LLF
CSB-BP404181LLF
CSB-MP404181LLF
CSB-EP404181LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L7/L12 (rplL) CSB-YP404182LLF
CSB-EP404182LLF
CSB-BP404182LLF
CSB-MP404182LLF
CSB-EP404182LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri HPr kinase/phosphorylase (hprK) CSB-YP404183LLF
CSB-EP404183LLF
CSB-BP404183LLF
CSB-MP404183LLF
CSB-EP404183LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Probable tRNA sulfurtransferase (thiI) CSB-YP404184LLF
CSB-EP404184LLF
CSB-BP404184LLF
CSB-MP404184LLF
CSB-EP404184LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Glutamate racemase (murI) CSB-YP404185LLF
CSB-EP404185LLF
CSB-BP404185LLF
CSB-MP404185LLF
CSB-EP404185LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S20 (rpsT) CSB-YP404186LLF
CSB-EP404186LLF
CSB-BP404186LLF
CSB-MP404186LLF
CSB-EP404186LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri GTPase obg (obg) CSB-YP404187LLF
CSB-EP404187LLF
CSB-BP404187LLF
CSB-MP404187LLF
CSB-EP404187LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Uridylate kinase (pyrH) CSB-YP404188LLF
CSB-EP404188LLF
CSB-BP404188LLF
CSB-MP404188LLF
CSB-EP404188LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA polymerase III polC-type (polC), partial CSB-YP404189LLF
CSB-EP404189LLF
CSB-BP404189LLF
CSB-MP404189LLF
CSB-EP404189LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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