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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Roseiflexus sp. Bifunctional protein FolD (folD) CSB-YP404150RIQ
CSB-EP404150RIQ
CSB-BP404150RIQ
CSB-MP404150RIQ
CSB-EP404150RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP404151SUF
CSB-EP404151SUF
CSB-BP404151SUF
CSB-MP404151SUF
CSB-EP404151SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Probable transaldolase (tal) CSB-YP404152SUF
CSB-EP404152SUF
CSB-BP404152SUF
CSB-MP404152SUF
CSB-EP404152SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Enolase (eno) CSB-YP404153SUF
CSB-EP404153SUF
CSB-BP404153SUF
CSB-MP404153SUF
CSB-EP404153SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L31 (rpmE) CSB-YP404154SUF
CSB-EP404154SUF
CSB-BP404154SUF
CSB-MP404154SUF
CSB-EP404154SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii UPF0102 protein Swit_0572 (Swit_0572) CSB-YP404155SUF
CSB-EP404155SUF
CSB-BP404155SUF
CSB-MP404155SUF
CSB-EP404155SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Aspartate carbamoyltransferase (pyrB) CSB-YP404156SUF
CSB-EP404156SUF
CSB-BP404156SUF
CSB-MP404156SUF
CSB-EP404156SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 5'-nucleotidase surE (surE) CSB-YP404157SUF
CSB-EP404157SUF
CSB-BP404157SUF
CSB-MP404157SUF
CSB-EP404157SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP404158SUF1
CSB-EP404158SUF1
CSB-BP404158SUF1
CSB-MP404158SUF1
CSB-EP404158SUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S11 (rpsK) CSB-YP404159SUF
CSB-EP404159SUF
CSB-BP404159SUF
CSB-MP404159SUF
CSB-EP404159SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S8 (rpsH) CSB-YP404160SUF
CSB-EP404160SUF
CSB-BP404160SUF
CSB-MP404160SUF
CSB-EP404160SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L3 (rplC) CSB-YP404161SUF
CSB-EP404161SUF
CSB-BP404161SUF
CSB-MP404161SUF
CSB-EP404161SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Bifunctional protein FolD 2 (folD2) CSB-YP404162SUF
CSB-EP404162SUF
CSB-BP404162SUF
CSB-MP404162SUF
CSB-EP404162SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP404163SUF
CSB-EP404163SUF
CSB-BP404163SUF
CSB-MP404163SUF
CSB-EP404163SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP404164SUF
CSB-EP404164SUF
CSB-BP404164SUF
CSB-MP404164SUF
CSB-EP404164SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S16 (rpsP) CSB-YP404165SUF
CSB-EP404165SUF
CSB-BP404165SUF
CSB-MP404165SUF
CSB-EP404165SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP404166SUF
CSB-EP404166SUF
CSB-BP404166SUF
CSB-MP404166SUF
CSB-EP404166SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Putative phosphotransferase Swit_2832 (Swit_2832) CSB-YP404167SUF
CSB-EP404167SUF
CSB-BP404167SUF
CSB-MP404167SUF
CSB-EP404167SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP404168SUF
CSB-EP404168SUF
CSB-BP404168SUF
CSB-MP404168SUF
CSB-EP404168SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP404169SUF
CSB-EP404169SUF
CSB-BP404169SUF
CSB-MP404169SUF
CSB-EP404169SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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