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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella ovis Modification methylase BabI (ccrM) CSB-YP404470BPI
CSB-EP404470BPI
CSB-BP404470BPI
CSB-MP404470BPI
CSB-EP404470BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis GTP pyrophosphokinase rsh (rsh), partial CSB-YP404471BPI
CSB-EP404471BPI
CSB-BP404471BPI
CSB-MP404471BPI
CSB-EP404471BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Phosphate acyltransferase (plsX) CSB-YP404472BPI
CSB-EP404472BPI
CSB-BP404472BPI
CSB-MP404472BPI
CSB-EP404472BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP404473BPI
CSB-EP404473BPI
CSB-BP404473BPI
CSB-MP404473BPI
CSB-EP404473BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP404474BPI1
CSB-EP404474BPI1
CSB-BP404474BPI1
CSB-MP404474BPI1
CSB-EP404474BPI1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP404475BPI
CSB-EP404475BPI
CSB-BP404475BPI
CSB-MP404475BPI
CSB-EP404475BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP404476BPI
CSB-EP404476BPI
CSB-BP404476BPI
CSB-MP404476BPI
CSB-EP404476BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Flavoprotein wrbA (wrbA) CSB-YP404477BPI
CSB-EP404477BPI
CSB-BP404477BPI
CSB-MP404477BPI
CSB-EP404477BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Protein hfq (hfq) CSB-YP404478BPI
CSB-EP404478BPI
CSB-BP404478BPI
CSB-MP404478BPI
CSB-EP404478BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP404479BPI
CSB-EP404479BPI
CSB-BP404479BPI
CSB-MP404479BPI
CSB-EP404479BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Alanine--tRNA ligase (alaS), partial CSB-YP404480BPI
CSB-EP404480BPI
CSB-BP404480BPI
CSB-MP404480BPI
CSB-EP404480BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S5 (rpsE) CSB-YP404481BPI
CSB-EP404481BPI
CSB-BP404481BPI
CSB-MP404481BPI
CSB-EP404481BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L5 (rplE) CSB-YP404482BPI
CSB-EP404482BPI
CSB-BP404482BPI
CSB-MP404482BPI
CSB-EP404482BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S3 (rpsC) CSB-YP404483BPI
CSB-EP404483BPI
CSB-BP404483BPI
CSB-MP404483BPI
CSB-EP404483BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L7/L12 (rplL) CSB-YP404484BPI
CSB-EP404484BPI
CSB-BP404484BPI
CSB-MP404484BPI
CSB-EP404484BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis UPF0303 protein BOV_1367 (BOV_1367) CSB-YP404485BPI
CSB-EP404485BPI
CSB-BP404485BPI
CSB-MP404485BPI
CSB-EP404485BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Trans-aconitate 2-methyltransferase (tam) CSB-YP404486BPI
CSB-EP404486BPI
CSB-BP404486BPI
CSB-MP404486BPI
CSB-EP404486BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis tRNA (Ile)-lysidine synthase CSB-YP404487BPI
CSB-EP404487BPI
CSB-BP404487BPI
CSB-MP404487BPI
CSB-EP404487BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L31 (rpmE) CSB-YP404488BPI
CSB-EP404488BPI
CSB-BP404488BPI
CSB-MP404488BPI
CSB-EP404488BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis ATP synthase gamma chain (atpG) CSB-YP404489BPI
CSB-EP404489BPI
CSB-BP404489BPI
CSB-MP404489BPI
CSB-EP404489BPI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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