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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sphingomonas wittichii Uridylate kinase (pyrH) CSB-YP404410SUF
CSB-EP404410SUF
CSB-BP404410SUF
CSB-MP404410SUF
CSB-EP404410SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Dihydrodipicolinate synthase (dapA) CSB-YP404411SUF
CSB-EP404411SUF
CSB-BP404411SUF
CSB-MP404411SUF
CSB-EP404411SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP404412SUF
CSB-EP404412SUF
CSB-BP404412SUF
CSB-MP404412SUF
CSB-EP404412SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP404413SUF
CSB-EP404413SUF
CSB-BP404413SUF
CSB-MP404413SUF
CSB-EP404413SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Valine--tRNA ligase (valS), partial CSB-YP404414SUF
CSB-EP404414SUF
CSB-BP404414SUF
CSB-MP404414SUF
CSB-EP404414SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Chaperone protein DnaK (dnaK), partial CSB-YP404415SUF
CSB-EP404415SUF
CSB-BP404415SUF
CSB-MP404415SUF
CSB-EP404415SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L17 (rplQ) CSB-YP404416SUF
CSB-EP404416SUF
CSB-BP404416SUF
CSB-MP404416SUF
CSB-EP404416SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L18 (rplR) CSB-YP404417SUF
CSB-EP404417SUF
CSB-BP404417SUF
CSB-MP404417SUF
CSB-EP404417SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L5 (rplE) CSB-YP404418SUF
CSB-EP404418SUF
CSB-BP404418SUF
CSB-MP404418SUF
CSB-EP404418SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 30S ribosomal protein S17 (rpsQ) CSB-YP404419SUF
CSB-EP404419SUF
CSB-BP404419SUF
CSB-MP404419SUF
CSB-EP404419SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L2 (rplB) CSB-YP404420SUF
CSB-EP404420SUF
CSB-BP404420SUF
CSB-MP404420SUF
CSB-EP404420SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Putative pterin-4-alpha-carbinolamine dehydratase (Swit_1419) CSB-YP404421SUF
CSB-EP404421SUF
CSB-BP404421SUF
CSB-MP404421SUF
CSB-EP404421SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP404422SUF
CSB-EP404422SUF
CSB-BP404422SUF
CSB-MP404422SUF
CSB-EP404422SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L28 (rpmB) CSB-YP404423SUF
CSB-EP404423SUF
CSB-BP404423SUF
CSB-MP404423SUF
CSB-EP404423SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii 50S ribosomal protein L20 (rplT) CSB-YP404424SUF
CSB-EP404424SUF
CSB-BP404424SUF
CSB-MP404424SUF
CSB-EP404424SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii UPF0301 protein Swit_2673 (Swit_2673) CSB-YP404425SUF
CSB-EP404425SUF
CSB-BP404425SUF
CSB-MP404425SUF
CSB-EP404425SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Tryptophan synthase alpha chain (trpA) CSB-YP404426SUF
CSB-EP404426SUF
CSB-BP404426SUF
CSB-MP404426SUF
CSB-EP404426SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii tRNA modification GTPase MnmE (mnmE) CSB-YP404427SUF
CSB-EP404427SUF
CSB-BP404427SUF
CSB-MP404427SUF
CSB-EP404427SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii Biotin synthase (bioB) CSB-YP404428SUF
CSB-EP404428SUF
CSB-BP404428SUF
CSB-MP404428SUF
CSB-EP404428SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphingomonas wittichii NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP404429SUF
CSB-EP404429SUF
CSB-BP404429SUF
CSB-MP404429SUF
CSB-EP404429SUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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