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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodomonas salina 50S ribosomal protein L3, chloroplastic (rpl3) CSB-YP405631RIK
CSB-EP405631RIK
CSB-BP405631RIK
CSB-MP405631RIK
CSB-EP405631RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP405632RIK
CSB-EP405632RIK
CSB-BP405632RIK
CSB-MP405632RIK
CSB-EP405632RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina 30S ribosomal protein S5, chloroplastic (rps5) CSB-YP405633RIK
CSB-EP405633RIK
CSB-BP405633RIK
CSB-MP405633RIK
CSB-EP405633RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina Protein psbN (psbN), partial CSB-YP405634RIK
CSB-EP405634RIK
CSB-BP405634RIK
CSB-MP405634RIK
CSB-EP405634RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein translation factor SUI1 homolog (GOS2) CSB-YP405635OFF
CSB-EP405635OFF
CSB-BP405635OFF
CSB-MP405635OFF
CSB-EP405635OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 150B (TMEM150B), partial CSB-YP405636HU1
CSB-EP405636HU1
CSB-BP405636HU1
CSB-MP405636HU1
CSB-EP405636HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein ENSP00000244321 CSB-YP405637HU
CSB-EP405637HU
CSB-BP405637HU
CSB-MP405637HU
CSB-EP405637HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tripartite motif-containing protein ENSP00000311270 CSB-YP405638HU
CSB-EP405638HU
CSB-BP405638HU
CSB-MP405638HU
CSB-EP405638HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative MAGE domain-containing protein MAGEA13P (MAGEA13P) CSB-YP405639HU
CSB-EP405639HU
CSB-BP405639HU
CSB-MP405639HU
CSB-EP405639HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tripartite motif-containing protein 43B (TRIM43B) CSB-YP405640HU
CSB-EP405640HU
CSB-BP405640HU
CSB-MP405640HU
CSB-EP405640HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative golgin subfamily A member 6-like protein 7 CSB-YP405641HU
CSB-EP405641HU
CSB-BP405641HU
CSB-MP405641HU
CSB-EP405641HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative nuclear envelope pore membrane protein POM 121B (POM121B), partial CSB-YP405642HU
CSB-EP405642HU
CSB-BP405642HU
CSB-MP405642HU
CSB-EP405642HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 235 (TMEM235), partial CSB-YP405643HU1
CSB-EP405643HU1
CSB-BP405643HU1
CSB-MP405643HU1
CSB-EP405643HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Coiled-coil domain-containing protein 42B (CCDC42B) CSB-YP405644HU
CSB-EP405644HU
CSB-BP405644HU
CSB-MP405644HU
CSB-EP405644HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative stereocilin-like protein (STRCP1), partial CSB-YP405645HU
CSB-EP405645HU
CSB-BP405645HU
CSB-MP405645HU
CSB-EP405645HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NPIP-like protein LOC729602 CSB-YP405646HU
CSB-EP405646HU
CSB-BP405646HU
CSB-MP405646HU
CSB-EP405646HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Aspartate carbamoyltransferase (pyrB) CSB-YP405647BPI
CSB-EP405647BPI
CSB-BP405647BPI
CSB-MP405647BPI
CSB-EP405647BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Bifunctional protein FolD (folD) CSB-YP405648BPI
CSB-EP405648BPI
CSB-BP405648BPI
CSB-MP405648BPI
CSB-EP405648BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP405649BPI
CSB-EP405649BPI
CSB-BP405649BPI
CSB-MP405649BPI
CSB-EP405649BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Undecaprenyl-diphosphatase (uppP), partial CSB-YP405650BPI
CSB-EP405650BPI
CSB-BP405650BPI
CSB-MP405650BPI
CSB-EP405650BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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