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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides vulgatus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP407232BOP
CSB-EP407232BOP
CSB-BP407232BOP
CSB-MP407232BOP
CSB-EP407232BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Ribonuclease Y (rny), partial CSB-YP407233BOP
CSB-EP407233BOP
CSB-BP407233BOP
CSB-MP407233BOP
CSB-EP407233BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP407234BOP
CSB-EP407234BOP
CSB-BP407234BOP
CSB-MP407234BOP
CSB-EP407234BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Chorismate synthase (aroC) CSB-YP407235BOP
CSB-EP407235BOP
CSB-BP407235BOP
CSB-MP407235BOP
CSB-EP407235BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP407236BOP
CSB-EP407236BOP
CSB-BP407236BOP
CSB-MP407236BOP
CSB-EP407236BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Altronate oxidoreductase (uxaB) CSB-YP407237BOP
CSB-EP407237BOP
CSB-BP407237BOP
CSB-MP407237BOP
CSB-EP407237BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP407238BOP
CSB-EP407238BOP
CSB-BP407238BOP
CSB-MP407238BOP
CSB-EP407238BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Probable transaldolase (tal) CSB-YP407239BOP
CSB-EP407239BOP
CSB-BP407239BOP
CSB-MP407239BOP
CSB-EP407239BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Thiazole synthase (thiG) CSB-YP407240BOP
CSB-EP407240BOP
CSB-BP407240BOP
CSB-MP407240BOP
CSB-EP407240BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Peptidyl-tRNA hydrolase (pth) CSB-YP407241BOP
CSB-EP407241BOP
CSB-BP407241BOP
CSB-MP407241BOP
CSB-EP407241BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP407242BOP
CSB-EP407242BOP
CSB-BP407242BOP
CSB-MP407242BOP
CSB-EP407242BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus LL-diaminopimelate aminotransferase (dapL) CSB-YP407243BOP
CSB-EP407243BOP
CSB-BP407243BOP
CSB-MP407243BOP
CSB-EP407243BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP407244BOP
CSB-EP407244BOP
CSB-BP407244BOP
CSB-MP407244BOP
CSB-EP407244BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Cytochrome c-552 (nrfA) CSB-YP407245PYB
CSB-EP407245PYB
CSB-BP407245PYB
CSB-MP407245PYB
CSB-EP407245PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP407246PYB
CSB-EP407246PYB
CSB-BP407246PYB
CSB-MP407246PYB
CSB-EP407246PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis LL-diaminopimelate aminotransferase (dapL) CSB-YP407247PYB
CSB-EP407247PYB
CSB-BP407247PYB
CSB-MP407247PYB
CSB-EP407247PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Putative Holliday junction resolvase (BDI_0655) CSB-YP407248PYB
CSB-EP407248PYB
CSB-BP407248PYB
CSB-MP407248PYB
CSB-EP407248PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis 50S ribosomal protein L35 (rpmI) CSB-YP407249PYB
CSB-EP407249PYB
CSB-BP407249PYB
CSB-MP407249PYB
CSB-EP407249PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Dihydrodipicolinate synthase (dapA) CSB-YP407250PYB
CSB-EP407250PYB
CSB-BP407250PYB
CSB-MP407250PYB
CSB-EP407250PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP407251PYB
CSB-EP407251PYB
CSB-BP407251PYB
CSB-MP407251PYB
CSB-EP407251PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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