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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae Thiazole synthase (thiG) CSB-YP410093KAX
CSB-EP410093KAX
CSB-BP410093KAX
CSB-MP410093KAX
CSB-EP410093KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Fumarate reductase subunit D (frdD), partial CSB-YP410094KAX1
CSB-EP410094KAX1
CSB-BP410094KAX1
CSB-MP410094KAX1
CSB-EP410094KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP410095KAX
CSB-EP410095KAX
CSB-BP410095KAX
CSB-MP410095KAX
CSB-EP410095KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae HTH-type transcriptional repressor NsrR (nsrR) CSB-YP410096KAX
CSB-EP410096KAX
CSB-BP410096KAX
CSB-MP410096KAX
CSB-EP410096KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L9 (rplI) CSB-YP410097KAX
CSB-EP410097KAX
CSB-BP410097KAX
CSB-MP410097KAX
CSB-EP410097KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0391 membrane protein KPN_04833 (KPN78578_47520), partial CSB-YP410098KAX
CSB-EP410098KAX
CSB-BP410098KAX
CSB-MP410098KAX
CSB-EP410098KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Na (+)/H (+) antiporter nhaA 2, partial CSB-YP410099KAX
CSB-EP410099KAX
CSB-BP410099KAX
CSB-MP410099KAX
CSB-EP410099KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Putative septation protein spoVG (spoVG) CSB-YP410100AUP
CSB-EP410100AUP
CSB-BP410100AUP
CSB-MP410100AUP
CSB-EP410100AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens ATP synthase epsilon chain (atpC) CSB-YP410101AUP
CSB-EP410101AUP
CSB-BP410101AUP
CSB-MP410101AUP
CSB-EP410101AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Glycerol kinase (glpK) CSB-YP410102AUP
CSB-EP410102AUP
CSB-BP410102AUP
CSB-MP410102AUP
CSB-EP410102AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP410103AUP
CSB-EP410103AUP
CSB-BP410103AUP
CSB-MP410103AUP
CSB-EP410103AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Protein translocase subunit SecA 1 (secA1), partial CSB-YP410104AUP
CSB-EP410104AUP
CSB-BP410104AUP
CSB-MP410104AUP
CSB-EP410104AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP410105AUP
CSB-EP410105AUP
CSB-BP410105AUP
CSB-MP410105AUP
CSB-EP410105AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Shikimate dehydrogenase (aroE) CSB-YP410106AUP
CSB-EP410106AUP
CSB-BP410106AUP
CSB-MP410106AUP
CSB-EP410106AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Catalase-peroxidase 1 (katG1), partial CSB-YP410107AUP
CSB-EP410107AUP
CSB-BP410107AUP
CSB-MP410107AUP
CSB-EP410107AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens NH (3)-dependent NAD (+) synthetase CSB-YP410108AUP
CSB-EP410108AUP
CSB-BP410108AUP
CSB-MP410108AUP
CSB-EP410108AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens GTPase obg (obg) CSB-YP410109AUP
CSB-EP410109AUP
CSB-BP410109AUP
CSB-MP410109AUP
CSB-EP410109AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP410110AUP
CSB-EP410110AUP
CSB-BP410110AUP
CSB-MP410110AUP
CSB-EP410110AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP410111AUP
CSB-EP410111AUP
CSB-BP410111AUP
CSB-MP410111AUP
CSB-EP410111AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Segregation and condensation protein B (scpB) CSB-YP410112AUP
CSB-EP410112AUP
CSB-BP410112AUP
CSB-MP410112AUP
CSB-EP410112AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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