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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Proline--tRNA ligase (proS), partial CSB-YP410153SUI
CSB-EP410153SUI
CSB-BP410153SUI
CSB-MP410153SUI
CSB-EP410153SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP410154SUI
CSB-EP410154SUI
CSB-BP410154SUI
CSB-MP410154SUI
CSB-EP410154SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Indole-3-glycerol phosphate synthase (trpC) CSB-YP410155SUI
CSB-EP410155SUI
CSB-BP410155SUI
CSB-MP410155SUI
CSB-EP410155SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP410156SUI
CSB-EP410156SUI
CSB-BP410156SUI
CSB-MP410156SUI
CSB-EP410156SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0398 protein SaurJH1_1535 (SaurJH1_1535) CSB-YP410157SUI
CSB-EP410157SUI
CSB-BP410157SUI
CSB-MP410157SUI
CSB-EP410157SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-dehydroquinate synthase (aroB) CSB-YP410158SUI
CSB-EP410158SUI
CSB-BP410158SUI
CSB-MP410158SUI
CSB-EP410158SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase Der (der) CSB-YP410159SUI
CSB-EP410159SUI
CSB-BP410159SUI
CSB-MP410159SUI
CSB-EP410159SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginine repressor (argR) CSB-YP410160SUI
CSB-EP410160SUI
CSB-BP410160SUI
CSB-MP410160SUI
CSB-EP410160SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative phosphotransferase SaurJH1_1655 (SaurJH1_1655) CSB-YP410161SUI
CSB-EP410161SUI
CSB-BP410161SUI
CSB-MP410161SUI
CSB-EP410161SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor 4 (lepA), partial CSB-YP410162SUI
CSB-EP410162SUI
CSB-BP410162SUI
CSB-MP410162SUI
CSB-EP410162SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP410163SUI
CSB-EP410163SUI
CSB-BP410163SUI
CSB-MP410163SUI
CSB-EP410163SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate--tRNA ligase (aspS), partial CSB-YP410164SUI
CSB-EP410164SUI
CSB-BP410164SUI
CSB-MP410164SUI
CSB-EP410164SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0735 ACT domain-containing protein SaurJH1_1733 (SaurJH1_1733) CSB-YP410165SUI
CSB-EP410165SUI
CSB-BP410165SUI
CSB-MP410165SUI
CSB-EP410165SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamyl-tRNA reductase (hemA) CSB-YP410166SUI
CSB-EP410166SUI
CSB-BP410166SUI
CSB-MP410166SUI
CSB-EP410166SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S4 (rpsD) CSB-YP410167SUI
CSB-EP410167SUI
CSB-BP410167SUI
CSB-MP410167SUI
CSB-EP410167SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0354 protein SaurJH1_1833 (SaurJH1_1833) CSB-YP410168SUI
CSB-EP410168SUI
CSB-BP410168SUI
CSB-MP410168SUI
CSB-EP410168SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine synthase (metK) CSB-YP410169SUI
CSB-EP410169SUI
CSB-BP410169SUI
CSB-MP410169SUI
CSB-EP410169SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splB (splB) CSB-YP410170SUI
CSB-EP410170SUI
CSB-BP410170SUI
CSB-MP410170SUI
CSB-EP410170SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Regulatory protein recX (recX) CSB-YP410171SUI
CSB-EP410171SUI
CSB-BP410171SUI
CSB-MP410171SUI
CSB-EP410171SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP410172SUI
CSB-EP410172SUI
CSB-BP410172SUI
CSB-MP410172SUI
CSB-EP410172SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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