Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP410633FLG
CSB-EP410633FLG
CSB-BP410633FLG
CSB-MP410633FLG
CSB-EP410633FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP410634FLG
CSB-EP410634FLG
CSB-BP410634FLG
CSB-MP410634FLG
CSB-EP410634FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional purine biosynthesis protein PurH (purH) CSB-YP410635FLG
CSB-EP410635FLG
CSB-BP410635FLG
CSB-MP410635FLG
CSB-EP410635FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphopantetheine adenylyltransferase (coaD) CSB-YP410636FLG
CSB-EP410636FLG
CSB-BP410636FLG
CSB-MP410636FLG
CSB-EP410636FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus MutS2 protein (mutS2), partial CSB-YP410637FLG
CSB-EP410637FLG
CSB-BP410637FLG
CSB-MP410637FLG
CSB-EP410637FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein MraZ (mraZ) CSB-YP410638FLG
CSB-EP410638FLG
CSB-BP410638FLG
CSB-MP410638FLG
CSB-EP410638FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate carbamoyltransferase (pyrB) CSB-YP410639FLG
CSB-EP410639FLG
CSB-BP410639FLG
CSB-MP410639FLG
CSB-EP410639FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphate acyltransferase (plsX) CSB-YP410640FLG
CSB-EP410640FLG
CSB-BP410640FLG
CSB-MP410640FLG
CSB-EP410640FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uridylate kinase (pyrH) CSB-YP410641FLG
CSB-EP410641FLG
CSB-BP410641FLG
CSB-MP410641FLG
CSB-EP410641FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S15 (rpsO) CSB-YP410642FLG
CSB-EP410642FLG
CSB-BP410642FLG
CSB-MP410642FLG
CSB-EP410642FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP410643FLG
CSB-EP410643FLG
CSB-BP410643FLG
CSB-MP410643FLG
CSB-EP410643FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Large-conductance mechanosensitive channel (mscL), partial CSB-YP410644FLG1
CSB-EP410644FLG1
CSB-BP410644FLG1
CSB-MP410644FLG1
CSB-EP410644FLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrodipicolinate synthase (dapA) CSB-YP410645FLG
CSB-EP410645FLG
CSB-BP410645FLG
CSB-MP410645FLG
CSB-EP410645FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Extracellular matrix-binding protein ebhA (ebhA), partial CSB-YP410646FLG
CSB-EP410646FLG
CSB-BP410646FLG
CSB-MP410646FLG
CSB-EP410646FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP410647FLG
CSB-EP410647FLG
CSB-BP410647FLG
CSB-MP410647FLG
CSB-EP410647FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elastin-binding protein ebpS (ebpS), partial CSB-YP410648FLG1
CSB-EP410648FLG1
CSB-BP410648FLG1
CSB-MP410648FLG1
CSB-EP410648FLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP410649FLG
CSB-EP410649FLG
CSB-BP410649FLG
CSB-MP410649FLG
CSB-EP410649FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0473 protein NWMN_1516 (NWMN_1516) CSB-YP410650FLG
CSB-EP410650FLG
CSB-BP410650FLG
CSB-MP410650FLG
CSB-EP410650FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP410651FLG
CSB-EP410651FLG
CSB-BP410651FLG
CSB-MP410651FLG
CSB-EP410651FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L27 (rpmA) CSB-YP410652FLG
CSB-EP410652FLG
CSB-BP410652FLG
CSB-MP410652FLG
CSB-EP410652FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>