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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Janthinobacterium sp. Multifunctional CCA protein (cca) CSB-YP411634JAQ
CSB-EP411634JAQ
CSB-BP411634JAQ
CSB-MP411634JAQ
CSB-EP411634JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Type III pantothenate kinase (coaX) CSB-YP411635JAQ
CSB-EP411635JAQ
CSB-BP411635JAQ
CSB-MP411635JAQ
CSB-EP411635JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP411636JAQ
CSB-EP411636JAQ
CSB-BP411636JAQ
CSB-MP411636JAQ
CSB-EP411636JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 30S ribosomal protein S11 (rpsK) CSB-YP411637JAQ
CSB-EP411637JAQ
CSB-BP411637JAQ
CSB-MP411637JAQ
CSB-EP411637JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 50S ribosomal protein L6 (rplF) CSB-YP411638JAQ
CSB-EP411638JAQ
CSB-BP411638JAQ
CSB-MP411638JAQ
CSB-EP411638JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 50S ribosomal protein L22 (rplV) CSB-YP411639JAQ
CSB-EP411639JAQ
CSB-BP411639JAQ
CSB-MP411639JAQ
CSB-EP411639JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 50S ribosomal protein L1 (rplA) CSB-YP411640JAQ
CSB-EP411640JAQ
CSB-BP411640JAQ
CSB-MP411640JAQ
CSB-EP411640JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Putative membrane protein insertion efficiency factor (mma_3695) CSB-YP411641JAQ
CSB-EP411641JAQ
CSB-BP411641JAQ
CSB-MP411641JAQ
CSB-EP411641JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP411642KAX
CSB-EP411642KAX
CSB-BP411642KAX
CSB-MP411642KAX
CSB-EP411642KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae RNA polymerase-associated protein RapA (rapA), partial CSB-YP411643KAX
CSB-EP411643KAX
CSB-BP411643KAX
CSB-MP411643KAX
CSB-EP411643KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Secretion monitor (secM) CSB-YP411644KAX
CSB-EP411644KAX
CSB-BP411644KAX
CSB-MP411644KAX
CSB-EP411644KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0231 protein KPN78578_01240 (KPN78578_01240) CSB-YP411645KAX
CSB-EP411645KAX
CSB-BP411645KAX
CSB-MP411645KAX
CSB-EP411645KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glutamyl-Q tRNA (Asp) synthetase CSB-YP411646KAX
CSB-EP411646KAX
CSB-BP411646KAX
CSB-MP411646KAX
CSB-EP411646KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP411647KAX
CSB-EP411647KAX
CSB-BP411647KAX
CSB-MP411647KAX
CSB-EP411647KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Na (+)-translocating NADH-quinone reductase subunit B, partial CSB-YP411648KAX
CSB-EP411648KAX
CSB-BP411648KAX
CSB-MP411648KAX
CSB-EP411648KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Xanthine phosphoribosyltransferase (gpt) CSB-YP411649KAX
CSB-EP411649KAX
CSB-BP411649KAX
CSB-MP411649KAX
CSB-EP411649KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Uncharacterized protein matC (matC) CSB-YP411650KAX
CSB-EP411650KAX
CSB-BP411650KAX
CSB-MP411650KAX
CSB-EP411650KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP411651KAX
CSB-EP411651KAX
CSB-BP411651KAX
CSB-MP411651KAX
CSB-EP411651KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L36 (rpmJ) CSB-YP411652KAX
CSB-EP411652KAX
CSB-BP411652KAX
CSB-MP411652KAX
CSB-EP411652KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0133 protein KPN78578_04440 (KPN78578_04440) CSB-YP411653KAX
CSB-EP411653KAX
CSB-BP411653KAX
CSB-MP411653KAX
CSB-EP411653KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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