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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Ceramide synthase subunit LIP1 (LIP1), partial CSB-YP411333STA
CSB-EP411333STA
CSB-BP411333STA
CSB-MP411333STA
CSB-EP411333STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ferric/cupric reductase transmembrane component 7 (FRE7), partial CSB-YP411334STA1
CSB-EP411334STA1
CSB-BP411334STA1
CSB-MP411334STA1
CSB-EP411334STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 39, mitochondrial (AIM39), partial CSB-YP411335STA
CSB-EP411335STA
CSB-BP411335STA
CSB-MP411335STA
CSB-EP411335STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Lipoyl synthase, mitochondrial (LIP5) CSB-YP411336STA
CSB-EP411336STA
CSB-BP411336STA
CSB-MP411336STA
CSB-EP411336STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Genetic interactor of prohibitins 3, mitochondrial (GEP3), partial CSB-YP411337STA
CSB-EP411337STA
CSB-BP411337STA
CSB-MP411337STA
CSB-EP411337STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Required for respiratory growth protein 7, mitochondrial (RRG7) CSB-YP411338STA
CSB-EP411338STA
CSB-BP411338STA
CSB-MP411338STA
CSB-EP411338STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP411339STA
CSB-EP411339STA
CSB-BP411339STA
CSB-MP411339STA
CSB-EP411339STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP411340STA
CSB-EP411340STA
CSB-BP411340STA
CSB-MP411340STA
CSB-EP411340STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy-related protein 27 (ATG27), partial CSB-YP411341STA
CSB-EP411341STA
CSB-BP411341STA
CSB-MP411341STA
CSB-EP411341STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae J protein JJJ2 (JJJ2), partial CSB-YP411342STA
CSB-EP411342STA
CSB-BP411342STA
CSB-MP411342STA
CSB-EP411342STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase eIF4A (TIF1) CSB-YP411343STA
CSB-EP411343STA
CSB-BP411343STA
CSB-MP411343STA
CSB-EP411343STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pheromone-regulated membrane protein 10 (PRM10), partial CSB-YP411344STA
CSB-EP411344STA
CSB-BP411344STA
CSB-MP411344STA
CSB-EP411344STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome-releasing factor 2, mitochondrial (MEF2), partial CSB-YP411345STA
CSB-EP411345STA
CSB-BP411345STA
CSB-MP411345STA
CSB-EP411345STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 37S ribosomal protein RSM18, mitochondrial (RSM18), partial CSB-YP411346STA
CSB-EP411346STA
CSB-BP411346STA
CSB-MP411346STA
CSB-EP411346STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 11 (AIM11), partial CSB-YP411347STA1
CSB-EP411347STA1
CSB-BP411347STA1
CSB-MP411347STA1
CSB-EP411347STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome biogenesis protein NSA2 (NSA2) CSB-YP411348STA
CSB-EP411348STA
CSB-BP411348STA
CSB-MP411348STA
CSB-EP411348STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Restriction of telomere capping protein 4 (RTC4) CSB-YP411349STA
CSB-EP411349STA
CSB-BP411349STA
CSB-MP411349STA
CSB-EP411349STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein SQS1 (SQS1), partial CSB-YP411350STA
CSB-EP411350STA
CSB-BP411350STA
CSB-MP411350STA
CSB-EP411350STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Required for respiratory growth protein 9, mitochondrial (RRG9) CSB-YP411351STA
CSB-EP411351STA
CSB-BP411351STA
CSB-MP411351STA
CSB-EP411351STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pro-apoptotic serine protease NMA111 (NMA111), partial CSB-YP411352STA
CSB-EP411352STA
CSB-BP411352STA
CSB-MP411352STA
CSB-EP411352STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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