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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus vannielii 30S ribosomal protein S9P (rps9p) CSB-YP412734MNS
CSB-EP412734MNS
CSB-BP412734MNS
CSB-MP412734MNS
CSB-EP412734MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Elongation factor 1-alpha (tuf) CSB-YP412735MNS
CSB-EP412735MNS
CSB-BP412735MNS
CSB-MP412735MNS
CSB-EP412735MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L32e (rpl32e) CSB-YP412736MNS
CSB-EP412736MNS
CSB-BP412736MNS
CSB-MP412736MNS
CSB-EP412736MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L15P (rpl15p) CSB-YP412737MNS
CSB-EP412737MNS
CSB-BP412737MNS
CSB-MP412737MNS
CSB-EP412737MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii UPF0278 protein Mevan_0782 (Mevan_0782) CSB-YP412738MNS
CSB-EP412738MNS
CSB-BP412738MNS
CSB-MP412738MNS
CSB-EP412738MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP412739MNS
CSB-EP412739MNS
CSB-BP412739MNS
CSB-MP412739MNS
CSB-EP412739MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP412740MNS
CSB-EP412740MNS
CSB-BP412740MNS
CSB-MP412740MNS
CSB-EP412740MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP412741MNS
CSB-EP412741MNS
CSB-BP412741MNS
CSB-MP412741MNS
CSB-EP412741MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 2-amino-5-formylamino-6-ribosylaminopyrimidin-4 (3H)-one 5'-monophosphate deformylase CSB-YP412742MNS
CSB-EP412742MNS
CSB-BP412742MNS
CSB-MP412742MNS
CSB-EP412742MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein LX (rplX) CSB-YP412743MNS
CSB-EP412743MNS
CSB-BP412743MNS
CSB-MP412743MNS
CSB-EP412743MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Putative transcriptional regulatory protein Mevan_1098 (Mevan_1098) CSB-YP412744MNS
CSB-EP412744MNS
CSB-BP412744MNS
CSB-MP412744MNS
CSB-EP412744MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP412745MNS
CSB-EP412745MNS
CSB-BP412745MNS
CSB-MP412745MNS
CSB-EP412745MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP412746MNS
CSB-EP412746MNS
CSB-BP412746MNS
CSB-MP412746MNS
CSB-EP412746MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Riboflavin kinase (ribK) CSB-YP412747MNS
CSB-EP412747MNS
CSB-BP412747MNS
CSB-MP412747MNS
CSB-EP412747MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Geranylgeranylglyceryl phosphate synthase (Mevan_1213) CSB-YP412748MNS
CSB-EP412748MNS
CSB-BP412748MNS
CSB-MP412748MNS
CSB-EP412748MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Prefoldin subunit beta (pfdB) CSB-YP412749MNS
CSB-EP412749MNS
CSB-BP412749MNS
CSB-MP412749MNS
CSB-EP412749MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Probable translation initiation factor IF-2 (infB), partial CSB-YP412750MNS
CSB-EP412750MNS
CSB-BP412750MNS
CSB-MP412750MNS
CSB-EP412750MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Shikimate kinase (aroK) CSB-YP412751MNS
CSB-EP412751MNS
CSB-BP412751MNS
CSB-MP412751MNS
CSB-EP412751MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Digeranylgeranylglycerophospholipid reductase (Mevan_1375) CSB-YP412752MNS
CSB-EP412752MNS
CSB-BP412752MNS
CSB-MP412752MNS
CSB-EP412752MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii GTP cyclohydrolase III (gch3) CSB-YP412753MNS
CSB-EP412753MNS
CSB-BP412753MNS
CSB-MP412753MNS
CSB-EP412753MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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