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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa 60 kDa chaperonin (groL), partial CSB-YP413574PZL
CSB-EP413574PZL
CSB-BP413574PZL
CSB-MP413574PZL
CSB-EP413574PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP413575PZL
CSB-EP413575PZL
CSB-BP413575PZL
CSB-MP413575PZL
CSB-EP413575PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S20 (rpsT) CSB-YP413576PZL
CSB-EP413576PZL
CSB-BP413576PZL
CSB-MP413576PZL
CSB-EP413576PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0114 protein PSPA7_5214 (PSPA7_5214), partial CSB-YP413577PZL1
CSB-EP413577PZL1
CSB-BP413577PZL1
CSB-MP413577PZL1
CSB-EP413577PZL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aspartate 1-decarboxylase (panD) CSB-YP413578PZL
CSB-EP413578PZL
CSB-BP413578PZL
CSB-MP413578PZL
CSB-EP413578PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein RnfH (rnfH) CSB-YP413579PZL
CSB-EP413579PZL
CSB-BP413579PZL
CSB-MP413579PZL
CSB-EP413579PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Urease subunit gamma (ureA) CSB-YP413580PZL
CSB-EP413580PZL
CSB-BP413580PZL
CSB-MP413580PZL
CSB-EP413580PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L9 (rplI) CSB-YP413581PZL
CSB-EP413581PZL
CSB-BP413581PZL
CSB-MP413581PZL
CSB-EP413581PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Shikimate kinase (aroK) CSB-YP413582PZL
CSB-EP413582PZL
CSB-BP413582PZL
CSB-MP413582PZL
CSB-EP413582PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP413583PZL
CSB-EP413583PZL
CSB-BP413583PZL
CSB-MP413583PZL
CSB-EP413583PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP413584PZL
CSB-EP413584PZL
CSB-BP413584PZL
CSB-MP413584PZL
CSB-EP413584PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L28 (rpmB) CSB-YP413585PZL
CSB-EP413585PZL
CSB-BP413585PZL
CSB-MP413585PZL
CSB-EP413585PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Bifunctional protein GlmU (glmU) CSB-YP413586PZL
CSB-EP413586PZL
CSB-BP413586PZL
CSB-MP413586PZL
CSB-EP413586PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP413587PZL
CSB-EP413587PZL
CSB-BP413587PZL
CSB-MP413587PZL
CSB-EP413587PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Type-2 serine--tRNA ligase (serS), partial CSB-YP413588MNR
CSB-EP413588MNR
CSB-BP413588MNR
CSB-MP413588MNR
CSB-EP413588MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Dihydrodipicolinate reductase (dapB) CSB-YP413589MNR
CSB-EP413589MNR
CSB-BP413589MNR
CSB-MP413589MNR
CSB-EP413589MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP413590MNR
CSB-EP413590MNR
CSB-BP413590MNR
CSB-MP413590MNR
CSB-EP413590MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tryptophan synthase alpha chain (trpA) CSB-YP413591MNR
CSB-EP413591MNR
CSB-BP413591MNR
CSB-MP413591MNR
CSB-EP413591MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable thymidylate kinase (tmk) CSB-YP413592MNR
CSB-EP413592MNR
CSB-BP413592MNR
CSB-MP413592MNR
CSB-EP413592MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase beta chain (atpB) CSB-YP413593MNR
CSB-EP413593MNR
CSB-BP413593MNR
CSB-MP413593MNR
CSB-EP413593MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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