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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial CSB-YP414574SUK1
CSB-EP414574SUK1
CSB-BP414574SUK1
CSB-MP414574SUK1
CSB-EP414574SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetylglutamate kinase (argB) CSB-YP414575SUK
CSB-EP414575SUK
CSB-BP414575SUK
CSB-MP414575SUK
CSB-EP414575SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-sulfur cluster repair protein ScdA (scdA) CSB-YP414576SUK
CSB-EP414576SUK
CSB-BP414576SUK
CSB-MP414576SUK
CSB-EP414576SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP414577SUK
CSB-EP414577SUK
CSB-BP414577SUK
CSB-MP414577SUK
CSB-EP414577SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Recombination protein RecR (recR) CSB-YP414578SUK
CSB-EP414578SUK
CSB-BP414578SUK
CSB-MP414578SUK
CSB-EP414578SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 33 kDa chaperonin (hslO) CSB-YP414579SUK
CSB-EP414579SUK
CSB-BP414579SUK
CSB-MP414579SUK
CSB-EP414579SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cysteine--tRNA ligase (cysS) CSB-YP414580SUK
CSB-EP414580SUK
CSB-BP414580SUK
CSB-MP414580SUK
CSB-EP414580SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribosomal protein L7Ae-like (SAHV_0542) CSB-YP414581SUK
CSB-EP414581SUK
CSB-BP414581SUK
CSB-MP414581SUK
CSB-EP414581SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribulokinase (araB), partial CSB-YP414582SUK
CSB-EP414582SUK
CSB-BP414582SUK
CSB-MP414582SUK
CSB-EP414582SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginine--tRNA ligase (argS), partial CSB-YP414583SUK
CSB-EP414583SUK
CSB-BP414583SUK
CSB-MP414583SUK
CSB-EP414583SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Undecaprenyl-diphosphatase (uppP) CSB-YP414584SUK
CSB-EP414584SUK
CSB-BP414584SUK
CSB-MP414584SUK
CSB-EP414584SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP414585SUK
CSB-EP414585SUK
CSB-BP414585SUK
CSB-MP414585SUK
CSB-EP414585SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative sporulation transcription regulator WhiA (whiA) CSB-YP414586SUK
CSB-EP414586SUK
CSB-BP414586SUK
CSB-MP414586SUK
CSB-EP414586SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-dehydroquinate dehydratase (aroD) CSB-YP414587SUK
CSB-EP414587SUK
CSB-BP414587SUK
CSB-MP414587SUK
CSB-EP414587SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--poly (phosphoribitol) ligase subunit 1 CSB-YP414588SUK
CSB-EP414588SUK
CSB-BP414588SUK
CSB-MP414588SUK
CSB-EP414588SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit D1, partial CSB-YP414589SUK
CSB-EP414589SUK
CSB-BP414589SUK
CSB-MP414589SUK
CSB-EP414589SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Adapter protein mecA (mecA) CSB-YP414590SUK
CSB-EP414590SUK
CSB-BP414590SUK
CSB-MP414590SUK
CSB-EP414590SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase (murE) CSB-YP414591SUK
CSB-EP414591SUK
CSB-BP414591SUK
CSB-MP414591SUK
CSB-EP414591SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP414592SUK
CSB-EP414592SUK
CSB-BP414592SUK
CSB-MP414592SUK
CSB-EP414592SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme A synthase (ctaA), partial CSB-YP414593SUK1
CSB-EP414593SUK1
CSB-BP414593SUK1
CSB-MP414593SUK1
CSB-EP414593SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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