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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype O:1b Uncharacterized protein YjeA (yjeA) CSB-YP413994YAN
CSB-EP413994YAN
CSB-BP413994YAN
CSB-MP413994YAN
CSB-EP413994YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b HTH-type transcriptional activator rhaR (rhaR) CSB-YP413995YAN
CSB-EP413995YAN
CSB-BP413995YAN
CSB-MP413995YAN
CSB-EP413995YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Chorismate--pyruvate lyase (ubiC) CSB-YP413996YAN
CSB-EP413996YAN
CSB-BP413996YAN
CSB-MP413996YAN
CSB-EP413996YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Thiamine-phosphate synthase (thiE) CSB-YP413997YAN
CSB-EP413997YAN
CSB-BP413997YAN
CSB-MP413997YAN
CSB-EP413997YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Elongation factor Tu 1 (tuf1) CSB-YP413998YAN
CSB-EP413998YAN
CSB-BP413998YAN
CSB-MP413998YAN
CSB-EP413998YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L17 (rplQ) CSB-YP413999YAN
CSB-EP413999YAN
CSB-BP413999YAN
CSB-MP413999YAN
CSB-EP413999YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L29 (rpmC) CSB-YP414000YAN
CSB-EP414000YAN
CSB-BP414000YAN
CSB-MP414000YAN
CSB-EP414000YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0270 protein YpsIP31758_3941 (YpsIP31758_3941) CSB-YP414001YAN
CSB-EP414001YAN
CSB-BP414001YAN
CSB-MP414001YAN
CSB-EP414001YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ferrous iron transport protein C (feoC) CSB-YP414002YAN
CSB-EP414002YAN
CSB-BP414002YAN
CSB-MP414002YAN
CSB-EP414002YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Glycogen synthase (glgA) CSB-YP414003YAN
CSB-EP414003YAN
CSB-BP414003YAN
CSB-MP414003YAN
CSB-EP414003YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP414004YAN
CSB-EP414004YAN
CSB-BP414004YAN
CSB-MP414004YAN
CSB-EP414004YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b DNA replication and repair protein recF (recF) CSB-YP414005YAN
CSB-EP414005YAN
CSB-BP414005YAN
CSB-MP414005YAN
CSB-EP414005YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP414006CWU
CSB-EP414006CWU
CSB-BP414006CWU
CSB-MP414006CWU
CSB-EP414006CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional protein GlmU (glmU) CSB-YP414007CWU
CSB-EP414007CWU
CSB-BP414007CWU
CSB-MP414007CWU
CSB-EP414007CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA modification GTPase MnmE (mnmE) CSB-YP414008CWU
CSB-EP414008CWU
CSB-BP414008CWU
CSB-MP414008CWU
CSB-EP414008CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Thiamine-phosphate synthase (thiE) CSB-YP414009CWU
CSB-EP414009CWU
CSB-BP414009CWU
CSB-MP414009CWU
CSB-EP414009CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP414010CWU
CSB-EP414010CWU
CSB-BP414010CWU
CSB-MP414010CWU
CSB-EP414010CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP414011CWU
CSB-EP414011CWU
CSB-BP414011CWU
CSB-MP414011CWU
CSB-EP414011CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative competence-damage inducible protein (cinA) CSB-YP414012CWU
CSB-EP414012CWU
CSB-BP414012CWU
CSB-MP414012CWU
CSB-EP414012CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartate 1-decarboxylase (panD) CSB-YP414013CWU
CSB-EP414013CWU
CSB-BP414013CWU
CSB-MP414013CWU
CSB-EP414013CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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