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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP414034CWU
CSB-EP414034CWU
CSB-BP414034CWU
CSB-MP414034CWU
CSB-EP414034CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP414035CWU
CSB-EP414035CWU
CSB-BP414035CWU
CSB-MP414035CWU
CSB-EP414035CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative sporulation transcription regulator WhiA (whiA) CSB-YP414036CWU
CSB-EP414036CWU
CSB-BP414036CWU
CSB-MP414036CWU
CSB-EP414036CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S4 B (rpsD2) CSB-YP414037CWU
CSB-EP414037CWU
CSB-BP414037CWU
CSB-MP414037CWU
CSB-EP414037CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP414038CWU
CSB-EP414038CWU
CSB-BP414038CWU
CSB-MP414038CWU
CSB-EP414038CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L6 (rplF) CSB-YP414039CWU
CSB-EP414039CWU
CSB-BP414039CWU
CSB-MP414039CWU
CSB-EP414039CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L29 (rpmC) CSB-YP414040CWU
CSB-EP414040CWU
CSB-BP414040CWU
CSB-MP414040CWU
CSB-EP414040CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S10 (rpsJ) CSB-YP414041CWU
CSB-EP414041CWU
CSB-BP414041CWU
CSB-MP414041CWU
CSB-EP414041CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L11 (rplK) CSB-YP414042CWU
CSB-EP414042CWU
CSB-BP414042CWU
CSB-MP414042CWU
CSB-EP414042CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Formate--tetrahydrofolate ligase (fhs), partial CSB-YP414043CWU
CSB-EP414043CWU
CSB-BP414043CWU
CSB-MP414043CWU
CSB-EP414043CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S6 (rpsF) CSB-YP414044CWU
CSB-EP414044CWU
CSB-BP414044CWU
CSB-MP414044CWU
CSB-EP414044CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Sugar fermentation stimulation protein homolog (sfsA) CSB-YP414045CWW
CSB-EP414045CWW
CSB-BP414045CWW
CSB-MP414045CWW
CSB-EP414045CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit c (atpE), partial CSB-YP414046CWW1
CSB-EP414046CWW1
CSB-BP414046CWW1
CSB-MP414046CWW1
CSB-EP414046CWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein translocase subunit SecA (secA), partial CSB-YP414047CWW
CSB-EP414047CWW
CSB-BP414047CWW
CSB-MP414047CWW
CSB-EP414047CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Enolase (eno) CSB-YP414048CWW
CSB-EP414048CWW
CSB-BP414048CWW
CSB-MP414048CWW
CSB-EP414048CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartate 1-decarboxylase (panD) CSB-YP414049CWW
CSB-EP414049CWW
CSB-BP414049CWW
CSB-MP414049CWW
CSB-EP414049CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D-alanine--D-alanine ligase (ddl) CSB-YP414050CWW
CSB-EP414050CWW
CSB-BP414050CWW
CSB-MP414050CWW
CSB-EP414050CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cobalamin synthase (cobS), partial CSB-YP414051CWW1
CSB-EP414051CWW1
CSB-BP414051CWW1
CSB-MP414051CWW1
CSB-EP414051CWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Xanthine phosphoribosyltransferase 2 (xpt2) CSB-YP414052CWW
CSB-EP414052CWW
CSB-BP414052CWW
CSB-MP414052CWW
CSB-EP414052CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative 3-methyladenine DNA glycosylase (CLI_1349) CSB-YP414053CWW
CSB-EP414053CWW
CSB-BP414053CWW
CSB-MP414053CWW
CSB-EP414053CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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