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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus subsp. cytotoxis Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP416114BOK
CSB-EP416114BOK
CSB-BP416114BOK
CSB-MP416114BOK
CSB-EP416114BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Thiazole synthase (thiG) CSB-YP416115BOK
CSB-EP416115BOK
CSB-BP416115BOK
CSB-MP416115BOK
CSB-EP416115BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0234 protein Bcer98_0876 (Bcer98_0876) CSB-YP416116BOK
CSB-EP416116BOK
CSB-BP416116BOK
CSB-MP416116BOK
CSB-EP416116BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Lactate utilization protein B (lutB) CSB-YP416117BOK
CSB-EP416117BOK
CSB-BP416117BOK
CSB-MP416117BOK
CSB-EP416117BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0753 protein Bcer98_1107 (Bcer98_1107), partial CSB-YP416118BOK
CSB-EP416118BOK
CSB-BP416118BOK
CSB-MP416118BOK
CSB-EP416118BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP416119BOK
CSB-EP416119BOK
CSB-BP416119BOK
CSB-MP416119BOK
CSB-EP416119BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP416120BOK
CSB-EP416120BOK
CSB-BP416120BOK
CSB-MP416120BOK
CSB-EP416120BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis GTPase Der (der) CSB-YP416121BOK
CSB-EP416121BOK
CSB-BP416121BOK
CSB-MP416121BOK
CSB-EP416121BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Histidinol-phosphate aminotransferase (hisC) CSB-YP416122BOK
CSB-EP416122BOK
CSB-BP416122BOK
CSB-MP416122BOK
CSB-EP416122BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Aspartate 1-decarboxylase (panD) CSB-YP416123BOK
CSB-EP416123BOK
CSB-BP416123BOK
CSB-MP416123BOK
CSB-EP416123BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Deoxyribose-phosphate aldolase (deoC) CSB-YP416124BOK
CSB-EP416124BOK
CSB-BP416124BOK
CSB-MP416124BOK
CSB-EP416124BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Sec-independent protein translocase protein TatA (tatA), partial CSB-YP416125BOK
CSB-EP416125BOK
CSB-BP416125BOK
CSB-MP416125BOK
CSB-EP416125BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0271 protein Bcer98_2060 (Bcer98_2060) CSB-YP416126BOK
CSB-EP416126BOK
CSB-BP416126BOK
CSB-MP416126BOK
CSB-EP416126BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Small, acid-soluble spore protein N (sspN) CSB-YP416127BOK
CSB-EP416127BOK
CSB-BP416127BOK
CSB-MP416127BOK
CSB-EP416127BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Holin-like protein CidA (cidA), partial CSB-YP416128BOK1
CSB-EP416128BOK1
CSB-BP416128BOK1
CSB-MP416128BOK1
CSB-EP416128BOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Protein RecA (recA) CSB-YP416129BOK
CSB-EP416129BOK
CSB-BP416129BOK
CSB-MP416129BOK
CSB-EP416129BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP416130BOK
CSB-EP416130BOK
CSB-BP416130BOK
CSB-MP416130BOK
CSB-EP416130BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Ribosome maturation factor RimM (rimM) CSB-YP416131BOK
CSB-EP416131BOK
CSB-BP416131BOK
CSB-MP416131BOK
CSB-EP416131BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP416132BOK
CSB-EP416132BOK
CSB-BP416132BOK
CSB-MP416132BOK
CSB-EP416132BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP416133BOK
CSB-EP416133BOK
CSB-BP416133BOK
CSB-MP416133BOK
CSB-EP416133BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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