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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum UPF0042 nucleotide-binding protein CLI_3561 (CLI_3561) CSB-YP416094CWW
CSB-EP416094CWW
CSB-BP416094CWW
CSB-MP416094CWW
CSB-EP416094CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphoglucosamine mutase (glmM) CSB-YP416095CWW
CSB-EP416095CWW
CSB-BP416095CWW
CSB-MP416095CWW
CSB-EP416095CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L36 (rpmJ) CSB-YP416096CWW
CSB-EP416096CWW
CSB-BP416096CWW
CSB-MP416096CWW
CSB-EP416096CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S8 (rpsH) CSB-YP416097CWW
CSB-EP416097CWW
CSB-BP416097CWW
CSB-MP416097CWW
CSB-EP416097CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L29 (rpmC) CSB-YP416098CWW
CSB-EP416098CWW
CSB-BP416098CWW
CSB-MP416098CWW
CSB-EP416098CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S10 (rpsJ) CSB-YP416099CWW
CSB-EP416099CWW
CSB-BP416099CWW
CSB-MP416099CWW
CSB-EP416099CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L7/L12 (rplL) CSB-YP416100CWW
CSB-EP416100CWW
CSB-BP416100CWW
CSB-MP416100CWW
CSB-EP416100CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP416101CWW
CSB-EP416101CWW
CSB-BP416101CWW
CSB-MP416101CWW
CSB-EP416101CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative septation protein spoVG (spoVG) CSB-YP416102CWW
CSB-EP416102CWW
CSB-BP416102CWW
CSB-MP416102CWW
CSB-EP416102CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative phosphotransferase CLI_3856 (CLI_3856) CSB-YP416103CWW
CSB-EP416103CWW
CSB-BP416103CWW
CSB-MP416103CWW
CSB-EP416103CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA modification GTPase MnmE (mnmE) CSB-YP416104CWW
CSB-EP416104CWW
CSB-BP416104CWW
CSB-MP416104CWW
CSB-EP416104CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP416105BOK
CSB-EP416105BOK
CSB-BP416105BOK
CSB-MP416105BOK
CSB-EP416105BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Lysine--tRNA ligase (lysS) CSB-YP416106BOK
CSB-EP416106BOK
CSB-BP416106BOK
CSB-MP416106BOK
CSB-EP416106BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP416107BOK
CSB-EP416107BOK
CSB-BP416107BOK
CSB-MP416107BOK
CSB-EP416107BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L23 (rplW) CSB-YP416108BOK
CSB-EP416108BOK
CSB-BP416108BOK
CSB-MP416108BOK
CSB-EP416108BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L16 (rplP) CSB-YP416109BOK
CSB-EP416109BOK
CSB-BP416109BOK
CSB-MP416109BOK
CSB-EP416109BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L18 (rplR) CSB-YP416110BOK
CSB-EP416110BOK
CSB-BP416110BOK
CSB-MP416110BOK
CSB-EP416110BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Adenylate kinase (adk) CSB-YP416111BOK
CSB-EP416111BOK
CSB-BP416111BOK
CSB-MP416111BOK
CSB-EP416111BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 30S ribosomal protein S9 (rpsI) CSB-YP416112BOK
CSB-EP416112BOK
CSB-BP416112BOK
CSB-MP416112BOK
CSB-EP416112BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP416113BOK
CSB-EP416113BOK
CSB-BP416113BOK
CSB-MP416113BOK
CSB-EP416113BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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