Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi 50S ribosomal protein L3 (rplC) CSB-YP417174VEA
CSB-EP417174VEA
CSB-BP417174VEA
CSB-MP417174VEA
CSB-EP417174VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi ATP-dependent protease subunit HslV (hslV) CSB-YP417175VEA
CSB-EP417175VEA
CSB-BP417175VEA
CSB-MP417175VEA
CSB-EP417175VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Multifunctional CCA protein (cca) CSB-YP417176VEA
CSB-EP417176VEA
CSB-BP417176VEA
CSB-MP417176VEA
CSB-EP417176VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Chaperone protein DnaJ (dnaJ) CSB-YP417177VEA
CSB-EP417177VEA
CSB-BP417177VEA
CSB-MP417177VEA
CSB-EP417177VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 8-amino-7-oxononanoate synthase (bioF) CSB-YP417178VEA
CSB-EP417178VEA
CSB-BP417178VEA
CSB-MP417178VEA
CSB-EP417178VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Uncharacterized protein YjeA (yjeA) CSB-YP417179VEA
CSB-EP417179VEA
CSB-BP417179VEA
CSB-MP417179VEA
CSB-EP417179VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP417180VEA
CSB-EP417180VEA
CSB-BP417180VEA
CSB-MP417180VEA
CSB-EP417180VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L11 (rplK) CSB-YP417181VEA
CSB-EP417181VEA
CSB-BP417181VEA
CSB-MP417181VEA
CSB-EP417181VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi RNA pyrophosphohydrolase (rppH) CSB-YP417182VEA
CSB-EP417182VEA
CSB-BP417182VEA
CSB-MP417182VEA
CSB-EP417182VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Protein translocase subunit SecA (secA), partial CSB-YP417183VEA
CSB-EP417183VEA
CSB-BP417183VEA
CSB-MP417183VEA
CSB-EP417183VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi tRNA (uracil (54)-C (5))-methyltransferase CSB-YP417184VEA
CSB-EP417184VEA
CSB-BP417184VEA
CSB-MP417184VEA
CSB-EP417184VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP417185VEA
CSB-EP417185VEA
CSB-BP417185VEA
CSB-MP417185VEA
CSB-EP417185VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Glutamate--tRNA ligase (gltX) CSB-YP417186VEA
CSB-EP417186VEA
CSB-BP417186VEA
CSB-MP417186VEA
CSB-EP417186VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP417187VEA
CSB-EP417187VEA
CSB-BP417187VEA
CSB-MP417187VEA
CSB-EP417187VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Protein syd (syd) CSB-YP417188VEA
CSB-EP417188VEA
CSB-BP417188VEA
CSB-MP417188VEA
CSB-EP417188VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0283 membrane protein VIBHAR_01918 (VIBHAR_01918), partial CSB-YP417189VEA1
CSB-EP417189VEA1
CSB-BP417189VEA1
CSB-MP417189VEA1
CSB-EP417189VEA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi tRNA (guanine-N (1)-)-methyltransferase CSB-YP417190VEA
CSB-EP417190VEA
CSB-BP417190VEA
CSB-MP417190VEA
CSB-EP417190VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Deoxyguanosinetriphosphate triphosphohydrolase-like protein (VIBHAR_01425) CSB-YP417191VEA
CSB-EP417191VEA
CSB-BP417191VEA
CSB-MP417191VEA
CSB-EP417191VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L35 (rpmI) CSB-YP417192VEA
CSB-EP417192VEA
CSB-BP417192VEA
CSB-MP417192VEA
CSB-EP417192VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Homoserine O-succinyltransferase (metA) CSB-YP417193VEA
CSB-EP417193VEA
CSB-BP417193VEA
CSB-MP417193VEA
CSB-EP417193VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>