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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cronobacter sakazakii Leucine--tRNA ligase (leuS), partial CSB-YP417154CXL
CSB-EP417154CXL
CSB-BP417154CXL
CSB-MP417154CXL
CSB-EP417154CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0271 protein ESA_02634 (ESA_02634) CSB-YP417155CXL
CSB-EP417155CXL
CSB-BP417155CXL
CSB-MP417155CXL
CSB-EP417155CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP417156CXL
CSB-EP417156CXL
CSB-BP417156CXL
CSB-MP417156CXL
CSB-EP417156CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Lysine--tRNA ligase (lysS), partial CSB-YP417157CXL
CSB-EP417157CXL
CSB-BP417157CXL
CSB-MP417157CXL
CSB-EP417157CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Probable intracellular septation protein A (VIBHAR_02768), partial CSB-YP417158VEA1
CSB-EP417158VEA1
CSB-BP417158VEA1
CSB-MP417158VEA1
CSB-EP417158VEA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Probable endonuclease 4 (nfo) CSB-YP417159VEA
CSB-EP417159VEA
CSB-BP417159VEA
CSB-MP417159VEA
CSB-EP417159VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP417160VEA
CSB-EP417160VEA
CSB-BP417160VEA
CSB-MP417160VEA
CSB-EP417160VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP417161VEA
CSB-EP417161VEA
CSB-BP417161VEA
CSB-MP417161VEA
CSB-EP417161VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP417162VEA
CSB-EP417162VEA
CSB-BP417162VEA
CSB-MP417162VEA
CSB-EP417162VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0745 protein VIBHAR_01387 (VIBHAR_01387) CSB-YP417163VEA
CSB-EP417163VEA
CSB-BP417163VEA
CSB-MP417163VEA
CSB-EP417163VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphoglycerate kinase (pgk) CSB-YP417164VEA
CSB-EP417164VEA
CSB-BP417164VEA
CSB-MP417164VEA
CSB-EP417164VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP417165VEA1
CSB-EP417165VEA1
CSB-BP417165VEA1
CSB-MP417165VEA1
CSB-EP417165VEA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Peptidase B (pepB) CSB-YP417166VEA
CSB-EP417166VEA
CSB-BP417166VEA
CSB-MP417166VEA
CSB-EP417166VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0352 protein VIBHAR_03027 (VIBHAR_03027) CSB-YP417167VEA
CSB-EP417167VEA
CSB-BP417167VEA
CSB-MP417167VEA
CSB-EP417167VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Integration host factor subunit beta (ihfB) CSB-YP417168VEA
CSB-EP417168VEA
CSB-BP417168VEA
CSB-MP417168VEA
CSB-EP417168VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Trigger factor (tig) CSB-YP417169VEA
CSB-EP417169VEA
CSB-BP417169VEA
CSB-MP417169VEA
CSB-EP417169VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Electron transport complex protein RnfB (rnfB) CSB-YP417170VEA
CSB-EP417170VEA
CSB-BP417170VEA
CSB-MP417170VEA
CSB-EP417170VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP417171VEA
CSB-EP417171VEA
CSB-BP417171VEA
CSB-MP417171VEA
CSB-EP417171VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Succinylglutamate desuccinylase (astE) CSB-YP417172VEA
CSB-EP417172VEA
CSB-BP417172VEA
CSB-MP417172VEA
CSB-EP417172VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP417173VEA
CSB-EP417173VEA
CSB-BP417173VEA
CSB-MP417173VEA
CSB-EP417173VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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