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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP418054CWU
CSB-EP418054CWU
CSB-BP418054CWU
CSB-MP418054CWU
CSB-EP418054CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Penicillin-binding protein 1A (pbpA), partial CSB-YP418055CWU
CSB-EP418055CWU
CSB-BP418055CWU
CSB-MP418055CWU
CSB-EP418055CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP418056CWU1
CSB-EP418056CWU1
CSB-BP418056CWU1
CSB-MP418056CWU1
CSB-EP418056CWU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP418057CWU
CSB-EP418057CWU
CSB-BP418057CWU
CSB-MP418057CWU
CSB-EP418057CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA ligase (ligA), partial CSB-YP418058CWU
CSB-EP418058CWU
CSB-BP418058CWU
CSB-MP418058CWU
CSB-EP418058CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Tyrosine--tRNA ligase (tyrS) CSB-YP418059CWU
CSB-EP418059CWU
CSB-BP418059CWU
CSB-MP418059CWU
CSB-EP418059CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UvrABC system protein B (uvrB), partial CSB-YP418060CWU
CSB-EP418060CWU
CSB-BP418060CWU
CSB-MP418060CWU
CSB-EP418060CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L36 (rpmJ) CSB-YP418061CWU
CSB-EP418061CWU
CSB-BP418061CWU
CSB-MP418061CWU
CSB-EP418061CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L5 (rplE) CSB-YP418062CWU
CSB-EP418062CWU
CSB-BP418062CWU
CSB-MP418062CWU
CSB-EP418062CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L2 (rplB) CSB-YP418063CWU
CSB-EP418063CWU
CSB-BP418063CWU
CSB-MP418063CWU
CSB-EP418063CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S7 (rpsG) CSB-YP418064CWU
CSB-EP418064CWU
CSB-BP418064CWU
CSB-MP418064CWU
CSB-EP418064CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cysteine--tRNA ligase (cysS) CSB-YP418065CWU
CSB-EP418065CWU
CSB-BP418065CWU
CSB-MP418065CWU
CSB-EP418065CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate racemase (murI) CSB-YP418066CWU
CSB-EP418066CWU
CSB-BP418066CWU
CSB-MP418066CWU
CSB-EP418066CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Chromosomal replication initiator protein DnaA (dnaA) CSB-YP418067CWW
CSB-EP418067CWW
CSB-BP418067CWW
CSB-MP418067CWW
CSB-EP418067CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP418068CWW
CSB-EP418068CWW
CSB-BP418068CWW
CSB-MP418068CWW
CSB-EP418068CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0313 protein CLI_0267 (CLI_0267), partial CSB-YP418069CWW
CSB-EP418069CWW
CSB-BP418069CWW
CSB-MP418069CWW
CSB-EP418069CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Adenine deaminase (ade), partial CSB-YP418070CWW
CSB-EP418070CWW
CSB-BP418070CWW
CSB-MP418070CWW
CSB-EP418070CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP418071CWW
CSB-EP418071CWW
CSB-BP418071CWW
CSB-MP418071CWW
CSB-EP418071CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA (guanine-N (7)-)-methyltransferase CSB-YP418072CWW
CSB-EP418072CWW
CSB-BP418072CWW
CSB-MP418072CWW
CSB-EP418072CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cobyric acid synthase (cobQ) CSB-YP418073CWW
CSB-EP418073CWW
CSB-BP418073CWW
CSB-MP418073CWW
CSB-EP418073CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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