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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Campylobacter curvus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP418854CWB
CSB-EP418854CWB
CSB-BP418854CWB
CSB-MP418854CWB
CSB-EP418854CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus UPF0234 protein Ccur92_01650 (Ccur92_01650) CSB-YP418855CWB
CSB-EP418855CWB
CSB-BP418855CWB
CSB-MP418855CWB
CSB-EP418855CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP418856CWB
CSB-EP418856CWB
CSB-BP418856CWB
CSB-MP418856CWB
CSB-EP418856CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP418857CWB
CSB-EP418857CWB
CSB-BP418857CWB
CSB-MP418857CWB
CSB-EP418857CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 30S ribosomal protein S18 (rpsR) CSB-YP418858CWB
CSB-EP418858CWB
CSB-BP418858CWB
CSB-MP418858CWB
CSB-EP418858CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP418859CWB
CSB-EP418859CWB
CSB-BP418859CWB
CSB-MP418859CWB
CSB-EP418859CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Na (+)/H (+) antiporter nhaA, partial CSB-YP418860CWB
CSB-EP418860CWB
CSB-BP418860CWB
CSB-MP418860CWB
CSB-EP418860CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L9 (rplI) CSB-YP418861CWB
CSB-EP418861CWB
CSB-BP418861CWB
CSB-MP418861CWB
CSB-EP418861CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Protein translocase subunit SecA (secA), partial CSB-YP418862CWB
CSB-EP418862CWB
CSB-BP418862CWB
CSB-MP418862CWB
CSB-EP418862CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Putative membrane protein insertion efficiency factor (Ccur92_11320) CSB-YP418863CWB
CSB-EP418863CWB
CSB-BP418863CWB
CSB-MP418863CWB
CSB-EP418863CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus SsrA-binding protein (smpB) CSB-YP418864CWB
CSB-EP418864CWB
CSB-BP418864CWB
CSB-MP418864CWB
CSB-EP418864CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Pantothenate synthetase (panC) CSB-YP418865CWB
CSB-EP418865CWB
CSB-BP418865CWB
CSB-MP418865CWB
CSB-EP418865CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 30S ribosomal protein S12 (rpsL) CSB-YP418866CWB
CSB-EP418866CWB
CSB-BP418866CWB
CSB-MP418866CWB
CSB-EP418866CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus UPF0763 protein Ccur92_13550 (Ccur92_13550) CSB-YP418867CWB
CSB-EP418867CWB
CSB-BP418867CWB
CSB-MP418867CWB
CSB-EP418867CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Alanine racemase (alr) CSB-YP418868CWB
CSB-EP418868CWB
CSB-BP418868CWB
CSB-MP418868CWB
CSB-EP418868CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L27 (rpmA) CSB-YP418869CWB
CSB-EP418869CWB
CSB-BP418869CWB
CSB-MP418869CWB
CSB-EP418869CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Uracil phosphoribosyltransferase (upp) CSB-YP418870CWB
CSB-EP418870CWB
CSB-BP418870CWB
CSB-MP418870CWB
CSB-EP418870CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Translation initiation factor IF-1 (infA) CSB-YP418871CWB
CSB-EP418871CWB
CSB-BP418871CWB
CSB-MP418871CWB
CSB-EP418871CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L6 (rplF) CSB-YP418872CWB
CSB-EP418872CWB
CSB-BP418872CWB
CSB-MP418872CWB
CSB-EP418872CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L16 (rplP) CSB-YP418873CWB
CSB-EP418873CWB
CSB-BP418873CWB
CSB-MP418873CWB
CSB-EP418873CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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