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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Roseiflexus castenholzii 30S ribosomal protein S16 (rpsP) CSB-YP419234RIP
CSB-EP419234RIP
CSB-BP419234RIP
CSB-MP419234RIP
CSB-EP419234RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Tryptophan synthase alpha chain (trpA) CSB-YP419235RIP
CSB-EP419235RIP
CSB-BP419235RIP
CSB-MP419235RIP
CSB-EP419235RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP419236RIP
CSB-EP419236RIP
CSB-BP419236RIP
CSB-MP419236RIP
CSB-EP419236RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Bifunctional protein pyrR (pyrR) CSB-YP419237RIP
CSB-EP419237RIP
CSB-BP419237RIP
CSB-MP419237RIP
CSB-EP419237RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii tRNA modification GTPase MnmE (mnmE) CSB-YP419238RIP
CSB-EP419238RIP
CSB-BP419238RIP
CSB-MP419238RIP
CSB-EP419238RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribose-5-phosphate isomerase A (rpiA) CSB-YP419239RIP
CSB-EP419239RIP
CSB-BP419239RIP
CSB-MP419239RIP
CSB-EP419239RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L33 (rpmG) CSB-YP419240RIP
CSB-EP419240RIP
CSB-BP419240RIP
CSB-MP419240RIP
CSB-EP419240RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 30S ribosomal protein S18 2 (rpsR2) CSB-YP419241RIP
CSB-EP419241RIP
CSB-BP419241RIP
CSB-MP419241RIP
CSB-EP419241RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L13 (rplM) CSB-YP419242RIP
CSB-EP419242RIP
CSB-BP419242RIP
CSB-MP419242RIP
CSB-EP419242RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Translation initiation factor IF-1 (infA) CSB-YP419243RIP
CSB-EP419243RIP
CSB-BP419243RIP
CSB-MP419243RIP
CSB-EP419243RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L14 (rplN) CSB-YP419244RIP
CSB-EP419244RIP
CSB-BP419244RIP
CSB-MP419244RIP
CSB-EP419244RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L23 (rplW) CSB-YP419245RIP
CSB-EP419245RIP
CSB-BP419245RIP
CSB-MP419245RIP
CSB-EP419245RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Uracil phosphoribosyltransferase (upp) CSB-YP419246RIP
CSB-EP419246RIP
CSB-BP419246RIP
CSB-MP419246RIP
CSB-EP419246RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L10 (rplJ) CSB-YP419247RIP
CSB-EP419247RIP
CSB-BP419247RIP
CSB-MP419247RIP
CSB-EP419247RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Chaperone protein DnaJ (dnaJ) CSB-YP419248RIP
CSB-EP419248RIP
CSB-BP419248RIP
CSB-MP419248RIP
CSB-EP419248RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera CASP-like protein VIT_14s0068g01400 (VIT_14s0068g01400), partial CSB-YP419249VFQ1
CSB-EP419249VFQ1
CSB-BP419249VFQ1
CSB-MP419249VFQ1
CSB-EP419249VFQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Probable polygalacturonase (GSVIVT00026920001, LOC100243180), partial CSB-YP419250VFQ1
CSB-EP419250VFQ1
CSB-BP419250VFQ1
CSB-MP419250VFQ1
CSB-EP419250VFQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera S-adenosylmethionine synthase 3 (METK3) CSB-YP419251VFQ
CSB-EP419251VFQ
CSB-BP419251VFQ
CSB-MP419251VFQ
CSB-EP419251VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Coiled-coil domain-containing protein 22 homolog (v1g180167), partial CSB-YP419252NGO
CSB-EP419252NGO
CSB-BP419252NGO
CSB-MP419252NGO
CSB-EP419252NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Nck-associated protein 1 homolog (napA), partial CSB-YP419253NGO
CSB-EP419253NGO
CSB-BP419253NGO
CSB-MP419253NGO
CSB-EP419253NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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