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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vanderwaltozyma polyspora Mitochondrial group I intron splicing factor CCM1 (CCM1), partial CSB-YP419274VDW
CSB-EP419274VDW
CSB-BP419274VDW
CSB-MP419274VDW
CSB-EP419274VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Altered inheritance of mitochondria protein 41, mitochondrial (AIM41) CSB-YP419275VDW
CSB-EP419275VDW
CSB-BP419275VDW
CSB-MP419275VDW
CSB-EP419275VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora 40S ribosomal protein S1 (RPS1) CSB-YP419276VDW
CSB-EP419276VDW
CSB-BP419276VDW
CSB-MP419276VDW
CSB-EP419276VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (Kpol_1073p15), partial CSB-YP419277VDW
CSB-EP419277VDW
CSB-BP419277VDW
CSB-MP419277VDW
CSB-EP419277VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora tRNA-dihydrouridine (47) synthase [NAD (P) (+)], partial CSB-YP419278VDW
CSB-EP419278VDW
CSB-BP419278VDW
CSB-MP419278VDW
CSB-EP419278VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ATPase synthesis protein 25, mitochondrial (ATP25), partial CSB-YP419279VDW
CSB-EP419279VDW
CSB-BP419279VDW
CSB-MP419279VDW
CSB-EP419279VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Protein SQS1 (SQS1), partial CSB-YP419280VDW
CSB-EP419280VDW
CSB-BP419280VDW
CSB-MP419280VDW
CSB-EP419280VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Altered inheritance of mitochondria protein 23, mitochondrial (AIM23) CSB-YP419281VDW
CSB-EP419281VDW
CSB-BP419281VDW
CSB-MP419281VDW
CSB-EP419281VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Histone H2A.Z-specific chaperone CHZ1 (CHZ1) CSB-YP419282VDW
CSB-EP419282VDW
CSB-BP419282VDW
CSB-MP419282VDW
CSB-EP419282VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Altered inheritance of mitochondria protein 3 (AIM3), partial CSB-YP419283VDW
CSB-EP419283VDW
CSB-BP419283VDW
CSB-MP419283VDW
CSB-EP419283VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Stationary phase protein 4 (SPG4) CSB-YP419284VDW
CSB-EP419284VDW
CSB-BP419284VDW
CSB-MP419284VDW
CSB-EP419284VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Actin cytoskeleton-regulatory complex protein PAN1 (PAN1), partial CSB-YP419285VDW
CSB-EP419285VDW
CSB-BP419285VDW
CSB-MP419285VDW
CSB-EP419285VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Selection and upkeep of intraepithelial T-cells protein 1 (Skint1), partial CSB-YP419286MO1
CSB-EP419286MO1
CSB-BP419286MO1
CSB-MP419286MO1
CSB-EP419286MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chrysochromulina ericina virus Major capsid protein (MCP), partial CSB-YP419287CWR
CSB-EP419287CWR
CSB-BP419287CWR
CSB-MP419287CWR
CSB-EP419287CWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ixodes sinensis Ixosin-B CSB-YP419288ILW
CSB-EP419288ILW
CSB-BP419288ILW
CSB-MP419288ILW
CSB-EP419288ILW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton equinum Carboxypeptidase S1 homolog A (SCPA), partial CSB-YP419289TPZ
CSB-EP419289TPZ
CSB-BP419289TPZ
CSB-MP419289TPZ
CSB-EP419289TPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (B9K17.150, NCU10468), partial CSB-YP419290NHA
CSB-EP419290NHA
CSB-BP419290NHA
CSB-MP419290NHA
CSB-EP419290NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine--tRNA ligase (serS) CSB-YP419291SUK
CSB-EP419291SUK
CSB-BP419291SUK
CSB-MP419291SUK
CSB-EP419291SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphopentomutase (deoB) CSB-YP419292SUK
CSB-EP419292SUK
CSB-BP419292SUK
CSB-MP419292SUK
CSB-EP419292SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus FMN-dependent NADH-azoreductase (azoR) CSB-YP419293SUK
CSB-EP419293SUK
CSB-BP419293SUK
CSB-MP419293SUK
CSB-EP419293SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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