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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O139:H28 Glutamine--tRNA ligase (glnS) CSB-YP419895EJD
CSB-EP419895EJD
CSB-BP419895EJD
CSB-MP419895EJD
CSB-EP419895EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP419896EJD
CSB-EP419896EJD
CSB-BP419896EJD
CSB-MP419896EJD
CSB-EP419896EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP419897EJD
CSB-EP419897EJD
CSB-BP419897EJD
CSB-MP419897EJD
CSB-EP419897EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Hydroxylamine reductase (hcp) CSB-YP419898EJD
CSB-EP419898EJD
CSB-BP419898EJD
CSB-MP419898EJD
CSB-EP419898EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Cytidylate kinase (cmk) CSB-YP419899EJD
CSB-EP419899EJD
CSB-BP419899EJD
CSB-MP419899EJD
CSB-EP419899EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Cell division inhibitor SulA (sulA) CSB-YP419900EJD
CSB-EP419900EJD
CSB-BP419900EJD
CSB-MP419900EJD
CSB-EP419900EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable malonic semialdehyde reductase RutE (rutE) CSB-YP419901EJD
CSB-EP419901EJD
CSB-BP419901EJD
CSB-MP419901EJD
CSB-EP419901EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glucans biosynthesis protein C (mdoC), partial CSB-YP419902EJD
CSB-EP419902EJD
CSB-BP419902EJD
CSB-MP419902EJD
CSB-EP419902EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Flagellar P-ring protein (flgI) CSB-YP419903EJD
CSB-EP419903EJD
CSB-BP419903EJD
CSB-MP419903EJD
CSB-EP419903EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0227 protein ycfP (ycfP) CSB-YP419904EJD
CSB-EP419904EJD
CSB-BP419904EJD
CSB-MP419904EJD
CSB-EP419904EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Cell division topological specificity factor (minE) CSB-YP419905EJD
CSB-EP419905EJD
CSB-BP419905EJD
CSB-MP419905EJD
CSB-EP419905EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Periplasmic trehalase (treA) CSB-YP419906EJD
CSB-EP419906EJD
CSB-BP419906EJD
CSB-MP419906EJD
CSB-EP419906EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Tryptophan synthase beta chain (trpB) CSB-YP419907EJD
CSB-EP419907EJD
CSB-BP419907EJD
CSB-MP419907EJD
CSB-EP419907EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP419908EJD
CSB-EP419908EJD
CSB-BP419908EJD
CSB-MP419908EJD
CSB-EP419908EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Transcriptional regulator lsrR (lsrR) CSB-YP419909EJD
CSB-EP419909EJD
CSB-BP419909EJD
CSB-MP419909EJD
CSB-EP419909EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0482 protein ynfB (ynfB) CSB-YP419910EJD
CSB-EP419910EJD
CSB-BP419910EJD
CSB-MP419910EJD
CSB-EP419910EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Transcriptional regulator slyA (slyA) CSB-YP419911EJD
CSB-EP419911EJD
CSB-BP419911EJD
CSB-MP419911EJD
CSB-EP419911EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Quinate/shikimate dehydrogenase (ydiB) CSB-YP419912EJD
CSB-EP419912EJD
CSB-BP419912EJD
CSB-MP419912EJD
CSB-EP419912EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L20 (rplT) CSB-YP419913EJD
CSB-EP419913EJD
CSB-BP419913EJD
CSB-MP419913EJD
CSB-EP419913EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP419914EJD
CSB-EP419914EJD
CSB-BP419914EJD
CSB-MP419914EJD
CSB-EP419914EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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