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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Citrobacter koseri Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP420095CWS
CSB-EP420095CWS
CSB-BP420095CWS
CSB-MP420095CWS
CSB-EP420095CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP420096CWS
CSB-EP420096CWS
CSB-BP420096CWS
CSB-MP420096CWS
CSB-EP420096CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0259 membrane protein CKO_01332 (CKO_01332) CSB-YP420097CWS
CSB-EP420097CWS
CSB-BP420097CWS
CSB-MP420097CWS
CSB-EP420097CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Gamma-aminobutyraldehyde dehydrogenase (prr) CSB-YP420098CWS
CSB-EP420098CWS
CSB-BP420098CWS
CSB-MP420098CWS
CSB-EP420098CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Voltage-gated ClC-type chloride channel ClcB (clcB), partial CSB-YP420099CWS
CSB-EP420099CWS
CSB-BP420099CWS
CSB-MP420099CWS
CSB-EP420099CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Electron transport complex protein RnfD (rnfD), partial CSB-YP420100CWS
CSB-EP420100CWS
CSB-BP420100CWS
CSB-MP420100CWS
CSB-EP420100CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Putative phosphotransferase CKO_01727 (CKO_01727) CSB-YP420101CWS
CSB-EP420101CWS
CSB-BP420101CWS
CSB-MP420101CWS
CSB-EP420101CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri NH (3)-dependent NAD (+) synthetase CSB-YP420102CWS
CSB-EP420102CWS
CSB-BP420102CWS
CSB-MP420102CWS
CSB-EP420102CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glucans biosynthesis glucosyltransferase H (mdoH), partial CSB-YP420103CWS
CSB-EP420103CWS
CSB-BP420103CWS
CSB-MP420103CWS
CSB-EP420103CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Chaperone modulatory protein CbpM (cbpM) CSB-YP420104CWS
CSB-EP420104CWS
CSB-BP420104CWS
CSB-MP420104CWS
CSB-EP420104CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP420105CWS
CSB-EP420105CWS
CSB-BP420105CWS
CSB-MP420105CWS
CSB-EP420105CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0434 protein CKO_02153 (CKO_02153) CSB-YP420106CWS
CSB-EP420106CWS
CSB-BP420106CWS
CSB-MP420106CWS
CSB-EP420106CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Translation initiation factor IF-1 (infA) CSB-YP420107CWS
CSB-EP420107CWS
CSB-BP420107CWS
CSB-MP420107CWS
CSB-EP420107CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP420108CWS
CSB-EP420108CWS
CSB-BP420108CWS
CSB-MP420108CWS
CSB-EP420108CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Formimidoylglutamase (hutG) CSB-YP420109CWS
CSB-EP420109CWS
CSB-BP420109CWS
CSB-MP420109CWS
CSB-EP420109CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Potassium-transporting ATPase A chain (kdpA), partial CSB-YP420110CWS
CSB-EP420110CWS
CSB-BP420110CWS
CSB-MP420110CWS
CSB-EP420110CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Bifunctional protein FolD (folD) CSB-YP420111CWS
CSB-EP420111CWS
CSB-BP420111CWS
CSB-MP420111CWS
CSB-EP420111CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 50S ribosomal protein L36 (rpmJ) CSB-YP420112CWS
CSB-EP420112CWS
CSB-BP420112CWS
CSB-MP420112CWS
CSB-EP420112CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP420113CWS
CSB-EP420113CWS
CSB-BP420113CWS
CSB-MP420113CWS
CSB-EP420113CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Anti-adapter protein iraP (iraP) CSB-YP420114CWS
CSB-EP420114CWS
CSB-BP420114CWS
CSB-MP420114CWS
CSB-EP420114CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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