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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salinispora arenicola Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP420796STC
CSB-EP420796STC
CSB-BP420796STC
CSB-MP420796STC
CSB-EP420796STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Dihydroorotate dehydrogenase (quinone) CSB-YP420797STC
CSB-EP420797STC
CSB-BP420797STC
CSB-MP420797STC
CSB-EP420797STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 50S ribosomal protein L20 (rplT) CSB-YP420798STC
CSB-EP420798STC
CSB-BP420798STC
CSB-MP420798STC
CSB-EP420798STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 60 kDa chaperonin 1 (groL1), partial CSB-YP420799STC
CSB-EP420799STC
CSB-BP420799STC
CSB-MP420799STC
CSB-EP420799STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP420800STC
CSB-EP420800STC
CSB-BP420800STC
CSB-MP420800STC
CSB-EP420800STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Probable rRNA maturation factor (Sare_3831) CSB-YP420801STC
CSB-EP420801STC
CSB-BP420801STC
CSB-MP420801STC
CSB-EP420801STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 50S ribosomal protein L27 (rpmA) CSB-YP420802STC
CSB-EP420802STC
CSB-BP420802STC
CSB-MP420802STC
CSB-EP420802STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 4-hydroxy-2-oxovalerate aldolase 1 (Sare_3900) CSB-YP420803STC
CSB-EP420803STC
CSB-BP420803STC
CSB-MP420803STC
CSB-EP420803STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Proteasome-associated ATPase (arc), partial CSB-YP420804STC
CSB-EP420804STC
CSB-BP420804STC
CSB-MP420804STC
CSB-EP420804STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP420805IND
CSB-EP420805IND
CSB-BP420805IND
CSB-MP420805IND
CSB-EP420805IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP420806IND
CSB-EP420806IND
CSB-BP420806IND
CSB-MP420806IND
CSB-EP420806IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP420807IND
CSB-EP420807IND
CSB-BP420807IND
CSB-MP420807IND
CSB-EP420807IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP420808IND
CSB-EP420808IND
CSB-BP420808IND
CSB-MP420808IND
CSB-EP420808IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP420809IND
CSB-EP420809IND
CSB-BP420809IND
CSB-MP420809IND
CSB-EP420809IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP420810IND
CSB-EP420810IND
CSB-BP420810IND
CSB-MP420810IND
CSB-EP420810IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP420811IND1
CSB-EP420811IND1
CSB-BP420811IND1
CSB-MP420811IND1
CSB-EP420811IND1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Tyrosyl-DNA phosphodiesterase 2 (TDP2) CSB-YP420812BO
CSB-EP420812BO
CSB-BP420812BO
CSB-MP420812BO
CSB-EP420812BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP420813BQD
CSB-EP420813BQD
CSB-BP420813BQD
CSB-MP420813BQD
CSB-EP420813BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP420814BQD
CSB-EP420814BQD
CSB-BP420814BQD
CSB-MP420814BQD
CSB-EP420814BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP420815BQD
CSB-EP420815BQD
CSB-BP420815BQD
CSB-MP420815BQD
CSB-EP420815BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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