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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus gordonii Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP421196FMN
CSB-EP421196FMN
CSB-BP421196FMN
CSB-MP421196FMN
CSB-EP421196FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Peptidase T (pepT) CSB-YP421197FMN
CSB-EP421197FMN
CSB-BP421197FMN
CSB-MP421197FMN
CSB-EP421197FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0342 protein SGO_1370 (SGO_1370) CSB-YP421198FMN
CSB-EP421198FMN
CSB-BP421198FMN
CSB-MP421198FMN
CSB-EP421198FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Enolase (eno) CSB-YP421199FMN
CSB-EP421199FMN
CSB-BP421199FMN
CSB-MP421199FMN
CSB-EP421199FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L11 (rplK) CSB-YP421200FMN
CSB-EP421200FMN
CSB-BP421200FMN
CSB-MP421200FMN
CSB-EP421200FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP421201FMN
CSB-EP421201FMN
CSB-BP421201FMN
CSB-MP421201FMN
CSB-EP421201FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Arginine deiminase (arcA) CSB-YP421202FMN
CSB-EP421202FMN
CSB-BP421202FMN
CSB-MP421202FMN
CSB-EP421202FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP421203FMN
CSB-EP421203FMN
CSB-BP421203FMN
CSB-MP421203FMN
CSB-EP421203FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Leucine--tRNA ligase (leuS), partial CSB-YP421204FMN
CSB-EP421204FMN
CSB-BP421204FMN
CSB-MP421204FMN
CSB-EP421204FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L17 (rplQ) CSB-YP421205FMN
CSB-EP421205FMN
CSB-BP421205FMN
CSB-MP421205FMN
CSB-EP421205FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L30 (rpmD) CSB-YP421206FMN
CSB-EP421206FMN
CSB-BP421206FMN
CSB-MP421206FMN
CSB-EP421206FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L14 (rplN) CSB-YP421207FMN
CSB-EP421207FMN
CSB-BP421207FMN
CSB-MP421207FMN
CSB-EP421207FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L23 (rplW) CSB-YP421208FMN
CSB-EP421208FMN
CSB-BP421208FMN
CSB-MP421208FMN
CSB-EP421208FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L33 (rpmG) CSB-YP421209FMN
CSB-EP421209FMN
CSB-BP421209FMN
CSB-MP421209FMN
CSB-EP421209FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP421210FMN
CSB-EP421210FMN
CSB-BP421210FMN
CSB-MP421210FMN
CSB-EP421210FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana pleuraden Pleurain-A3 CSB-YP421211RIF
CSB-EP421211RIF
CSB-BP421211RIF
CSB-MP421211RIF
CSB-EP421211RIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase (GL50803_12081), partial CSB-YP421212GFY
CSB-EP421212GFY
CSB-BP421212GFY
CSB-MP421212GFY
CSB-EP421212GFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Protein SEY1 homolog (GL50803_32509), partial CSB-YP421213GFY
CSB-EP421213GFY
CSB-BP421213GFY
CSB-MP421213GFY
CSB-EP421213GFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP421214ILV
CSB-EP421214ILV
CSB-BP421214ILV
CSB-MP421214ILV
CSB-EP421214ILV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP421215ILP
CSB-EP421215ILP
CSB-BP421215ILP
CSB-MP421215ILP
CSB-EP421215ILP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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