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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus pumilus 30S ribosomal protein S7 (rpsG) CSB-YP426479BOL
CSB-EP426479BOL
CSB-BP426479BOL
CSB-MP426479BOL
CSB-EP426479BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L23 (rplW) CSB-YP426480BOL
CSB-EP426480BOL
CSB-BP426480BOL
CSB-MP426480BOL
CSB-EP426480BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L14 (rplN) CSB-YP426481BOL
CSB-EP426481BOL
CSB-BP426481BOL
CSB-MP426481BOL
CSB-EP426481BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Translation initiation factor IF-1 (infA) CSB-YP426482BOL
CSB-EP426482BOL
CSB-BP426482BOL
CSB-MP426482BOL
CSB-EP426482BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Phosphoglucosamine mutase (glmM) CSB-YP426483BOL
CSB-EP426483BOL
CSB-BP426483BOL
CSB-MP426483BOL
CSB-EP426483BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus D-alanine--D-alanine ligase (ddl) CSB-YP426484BOL
CSB-EP426484BOL
CSB-BP426484BOL
CSB-MP426484BOL
CSB-EP426484BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Recombination protein RecR (recR) CSB-YP426485BOL
CSB-EP426485BOL
CSB-BP426485BOL
CSB-MP426485BOL
CSB-EP426485BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 2-isopropylmalate synthase 2 (leuA2), partial CSB-YP426486BOL
CSB-EP426486BOL
CSB-BP426486BOL
CSB-MP426486BOL
CSB-EP426486BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus DNA ligase (ligA), partial CSB-YP426487BOL
CSB-EP426487BOL
CSB-BP426487BOL
CSB-MP426487BOL
CSB-EP426487BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Ferredoxin--NADP reductase 1 (BPUM_0777) CSB-YP426488BOL
CSB-EP426488BOL
CSB-BP426488BOL
CSB-MP426488BOL
CSB-EP426488BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0736 protein BPUM_1067 (BPUM_1067) CSB-YP426489BOL
CSB-EP426489BOL
CSB-BP426489BOL
CSB-MP426489BOL
CSB-EP426489BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP426490BOL
CSB-EP426490BOL
CSB-BP426490BOL
CSB-MP426490BOL
CSB-EP426490BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0637 protein BPUM_1363 (BPUM_1363) CSB-YP426491BOL
CSB-EP426491BOL
CSB-BP426491BOL
CSB-MP426491BOL
CSB-EP426491BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Uncharacterized N-acetyltransferase BPUM_1403 (BPUM_1403) CSB-YP426492BOL
CSB-EP426492BOL
CSB-BP426492BOL
CSB-MP426492BOL
CSB-EP426492BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Cell division protein SepF (sepF) CSB-YP426493BOL
CSB-EP426493BOL
CSB-BP426493BOL
CSB-MP426493BOL
CSB-EP426493BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP426494BOL
CSB-EP426494BOL
CSB-BP426494BOL
CSB-MP426494BOL
CSB-EP426494BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Adenylyl-sulfate kinase (cysC) CSB-YP426495BOL
CSB-EP426495BOL
CSB-BP426495BOL
CSB-MP426495BOL
CSB-EP426495BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Ribonuclease HII (rnhB) CSB-YP426496BOL
CSB-EP426496BOL
CSB-BP426496BOL
CSB-MP426496BOL
CSB-EP426496BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP426497BOL
CSB-EP426497BOL
CSB-BP426497BOL
CSB-MP426497BOL
CSB-EP426497BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Protein RecA (recA) CSB-YP426498BOL
CSB-EP426498BOL
CSB-BP426498BOL
CSB-MP426498BOL
CSB-EP426498BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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