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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Thermotoga lettingae 50S ribosomal protein L14 (rplN) CSB-YP426459TKE
CSB-EP426459TKE
CSB-BP426459TKE
CSB-MP426459TKE
CSB-EP426459TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 30S ribosomal protein S4 (rpsD) CSB-YP426460TKE
CSB-EP426460TKE
CSB-BP426460TKE
CSB-MP426460TKE
CSB-EP426460TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP426461TKE
CSB-EP426461TKE
CSB-BP426461TKE
CSB-MP426461TKE
CSB-EP426461TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP426462TKE
CSB-EP426462TKE
CSB-BP426462TKE
CSB-MP426462TKE
CSB-EP426462TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Protein translocase subunit SecA (secA), partial CSB-YP426463TKE
CSB-EP426463TKE
CSB-BP426463TKE
CSB-MP426463TKE
CSB-EP426463TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Uridylate kinase (pyrH) CSB-YP426464TKE
CSB-EP426464TKE
CSB-BP426464TKE
CSB-MP426464TKE
CSB-EP426464TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Glycerol kinase (glpK) CSB-YP426465TKE
CSB-EP426465TKE
CSB-BP426465TKE
CSB-MP426465TKE
CSB-EP426465TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP426466TKE
CSB-EP426466TKE
CSB-BP426466TKE
CSB-MP426466TKE
CSB-EP426466TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae tRNA modification GTPase MnmE (mnmE) CSB-YP426467TKE
CSB-EP426467TKE
CSB-BP426467TKE
CSB-MP426467TKE
CSB-EP426467TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Probable septum site-determining protein MinC (minC) CSB-YP426468TKE
CSB-EP426468TKE
CSB-BP426468TKE
CSB-MP426468TKE
CSB-EP426468TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae UPF0296 protein Tlet_1629 (Tlet_1629) CSB-YP426469TKE
CSB-EP426469TKE
CSB-BP426469TKE
CSB-MP426469TKE
CSB-EP426469TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Aspartate--tRNA ligase (aspS), partial CSB-YP426470TKE
CSB-EP426470TKE
CSB-BP426470TKE
CSB-MP426470TKE
CSB-EP426470TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP426471TKE1
CSB-EP426471TKE1
CSB-BP426471TKE1
CSB-MP426471TKE1
CSB-EP426471TKE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP426472TKE
CSB-EP426472TKE
CSB-BP426472TKE
CSB-MP426472TKE
CSB-EP426472TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP426473TKE
CSB-EP426473TKE
CSB-BP426473TKE
CSB-MP426473TKE
CSB-EP426473TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP426474TKE
CSB-EP426474TKE
CSB-BP426474TKE
CSB-MP426474TKE
CSB-EP426474TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP426475TKE
CSB-EP426475TKE
CSB-BP426475TKE
CSB-MP426475TKE
CSB-EP426475TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L25 (rplY) CSB-YP426476BOL
CSB-EP426476BOL
CSB-BP426476BOL
CSB-MP426476BOL
CSB-EP426476BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP426477BOL
CSB-EP426477BOL
CSB-BP426477BOL
CSB-MP426477BOL
CSB-EP426477BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L10 (rplJ) CSB-YP426478BOL
CSB-EP426478BOL
CSB-BP426478BOL
CSB-MP426478BOL
CSB-EP426478BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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