多克隆抗體
抗體通常分為兩大類,分別是單克隆抗體和多克隆抗體。顧名思義,由一種抗原決定簇刺激機(jī)體,由一個(gè)B淋巴細(xì)胞接受該抗原刺激所產(chǎn)生的抗體稱之為單克隆抗體。由多種抗原決定簇刺激機(jī)體,相應(yīng)地就產(chǎn)生各種各樣的單克隆抗體,這些單克隆抗體混雜在一起就是多克隆抗體,機(jī)體內(nèi)所產(chǎn)生的抗體就是多克隆抗體。簡(jiǎn)言之,多克隆抗體就是用一種包含多種抗原決定簇的抗原免疫動(dòng)物,刺激機(jī)體多個(gè)B細(xì)胞克隆產(chǎn)生針對(duì)多種抗原表位的不同抗體后,所獲得的免疫血清實(shí)際上是含有多種抗體的混合物。
產(chǎn)品優(yōu)勢(shì)
華美生物從事抗體制備生產(chǎn)已有16年,擁有一批從事抗體研發(fā)制備多年的技術(shù)人員、先進(jìn)的抗體生產(chǎn)設(shè)備和技術(shù)。所有華美生物多克隆抗體都是由重組蛋白或天然蛋白制備得來的。與多肽制備的抗體相比,重組蛋白或天然蛋白制備的抗體親和力和特異性強(qiáng)、識(shí)別抗原位點(diǎn)多、應(yīng)用范圍更廣。
下面是部分多克隆抗體應(yīng)用展示圖:
CFHR5 Antibody (CSB-PA883624LA01HU)
STYX Antibody (CSB-PA851966LA01HU)
2-hydroxyisobutyryl-HIST1H1C (K109) Antibody (CSB-PA010378OA109hibHU)
2-hydroxyisobutyryl-HIST1H3A (K18) Antibody (CSB-PA010418PA18hibHU)
LGALS4 Antibody (CSB-PA012889LA01HU)
WDR73 Antibody (CSB-PA744047LA01HU)
TNFRSF1A Antibody (CSB-PA023977LA01HU)
UMODL1 Antibody (CSB-PA025617LA01HU)
產(chǎn)品列表
您可以在下述列表中尋找您所需要的產(chǎn)品,或直接在頂部搜索框中輸入您需要查找的靶點(diǎn)、產(chǎn)品名稱、貨號(hào)。有任何問題,請(qǐng)點(diǎn)擊此處在線咨詢。
如果您未找到所需要的產(chǎn)品,請(qǐng)點(diǎn)擊此處查看多克隆抗體定制服務(wù)。
產(chǎn)品名稱 | 貨號(hào) | 反應(yīng)種屬 | 應(yīng)用范圍 | 規(guī)格 |
---|---|---|---|---|
gag Antibody | CSB-PA356299XA01HKF | Human immunodeficiency virus type 1 group M subtype A (isolate MAL) (HIV-1) | ELISA, WB | 100μl/50μl/20μl |
gag Antibody | CSB-PA328493XA01HKG | Human immunodeficiency virus type 1 group M subtype A (isolate U455) (HIV-1) | ELISA, WB | 100μl/50μl/20μl |
MIP1 Antibody | CSB-PA322240XA01SVG | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB | 100μl/50μl/20μl |
Omega-hexatoxin-Hv1a Antibody | CSB-PA347842ZA01HAD | Hadronyche versuta | ELISA, WB | 100μl/50μl/20μl |
ompA Antibody | CSB-PA359226ZA01EOD | Escherichia coli O157:H7 | ELISA, WB | 100μl/50μl/20μl |
Metallo-beta-lactamase type 2 Antibody | CSB-PA346782ZA01SYN | Serratia marcescens | ELISA, WB | 100μl/50μl/20μl |
Abrin-a Antibody | CSB-PA319970ZA01AAC | Abrus precatorius | ELISA, WB | 100μl/50μl/20μl |
A27L Antibody | CSB-PA26789ZA01VAR | Variola virus | ELISA, WB | 100μl/50μl/20μl |
Barstar (Ribonuclease inhibitor) Antibody | CSB-PA320008ZA01BQB | Bacillus amyloliquefaciens | ELISA, WB | 100μl/50μl/20μl |
Genome polyprotein Antibody | CSB-PA312464ZA01BKX | Bovine viral diarrhea virus | ELISA, WB | 100μl/50μl/20μl |
ompX Antibody | CSB-PA359230ZA01EOD | Escherichia coli O157:H7 | ELISA, WB | 100μl/50μl/20μl |
Ribonuclease Antibody | CSB-PA360430ZA01BQB | Bacillus amyloliquefaciens | ELISA, WB | 100μl/50μl/20μl |
lprG Antibody | CSB-PA358685ZA01MVZ | Mycobacterium tuberculosis | ELISA, WB | 100μl/50μl/20μl |
Phospho-PLK4 (S305) Antibody | CSB-PA018196XA01phHU | Homo sapiens (Human) | ELISA | 100μl/50μl/20μl |
Phospho-mFLNc (S2237) Antibody | CSB-PA837745XA01phMO | Mus musculus (Mouse) | ELISA | 100μl/50μl/20μl |
BRO1 Antibody | CSB-PA798541XA01DOA | Arabidopsis thaliana | ELISA, WB | 100μl/50μl/20μl |
PDF2.3 Antibody | CSB-PA931869XA01DOA | Arabidopsis thaliana | ELISA, WB | 100μl/50μl/20μl |
DGAT3 Antibody | CSB-PA273566XA01DOA | Drosophila melanogaster (Fruit fly) | ELISA, WB | 100μl/50μl/20μl |
DREB2C Antibody | CSB-PA850687XA01DOA | Drosophila melanogaster (Fruit fly) | ELISA, WB | 100μl/50μl/20μl |
FEN1 Antibody | CSB-PA008585XA01DOA | Drosophila melanogaster (Fruit fly) | ELISA, WB | 100μl/50μl/20μl |
部分引用文獻(xiàn)
SLC25A47 is a novel determinant of hepatic mitochondrial function implicated in liver fibrosis. Journal of Hepatology, 2022. (IF=25.083)
引用產(chǎn)品:YME1L1 Antibody, CSB-PA026267GA01HU
An Image-Based High-Throughput and High-Content Drug Screening Method Based on Microarray and Expansion Microscopy. ACS nano, 2023. (IF=18.027)
引用產(chǎn)品: TOMM20 Antibody, CSB-PA618983ESR2HU
Extracellular vesicles derived from Akkermansia muciniphila promote placentation and mitigate preeclampsia in a mouse model. Journal of extracellular vesicles, 2023. (IF=17.337)
引用產(chǎn)品:ompA Antibody, CSB-PA706579LA01RMS
AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation. Nucleic acids research, 2021. (IF=16.973)
引用產(chǎn)品:CDKL3 Antibody, CSB-PA150181; GAPDH Antibody, CSB-PA00025A0Rb
Mitochondria supply sub-lethal signals for cytokine secretion and DNA-damage in H. pylori infection. cell death & differentiation, 2022. (IF=15.828)
引用產(chǎn)品:TIFA Antibody, CSB-PA839301LA01HU
點(diǎn)擊查看華美生物20,000+引用文獻(xiàn)
關(guān)于多抗
正如前面所提到的,多克隆抗體猶如許多單克隆抗體的集合,它們有的特異性高,有的特異性低,但總體特異性符合正態(tài)分布曲線的形式,而其特異性的總體表現(xiàn)也應(yīng)處于統(tǒng)計(jì)學(xué)正態(tài)曲線中位線的水平。與單克隆抗體相比,多克隆抗體的優(yōu)缺點(diǎn)如下:
優(yōu)勢(shì):
- 由于多克隆抗體具有多個(gè)表位結(jié)合位點(diǎn),可與靶蛋白的多個(gè)表位結(jié)合,所以,多克隆抗體有助于放大低表達(dá)水平的靶蛋白信號(hào),但是,從結(jié)果的準(zhǔn)確性來說,這個(gè)優(yōu)勢(shì)也是劣勢(shì),它會(huì)給定量實(shí)驗(yàn)造成不利影響,使結(jié)果變得不準(zhǔn)確。
- 由于能識(shí)別多個(gè)表位,多克隆抗體更適用于IP/ChIP實(shí)驗(yàn),可得到更好的結(jié)果。
- 制備生產(chǎn)時(shí)間短,所需技術(shù)和技能要求不高,成本比單克隆抗體低,穩(wěn)定性強(qiáng)。
- 雙抗夾心法ELISA中捕獲抗體首選多克隆抗體,可以快速捕獲靶蛋白。
劣勢(shì):
- 易于產(chǎn)生批次間差異。
- 可產(chǎn)生大量非特異性抗體,使應(yīng)用中背景信號(hào)強(qiáng)。
- 多表位使得檢測(cè)免疫原序列以確定是否有任何交叉反應(yīng)變得尤為重要。