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跨膜蛋白系列四:人白細(xì)胞抗原(HLA)

什么是人類白細(xì)胞抗原?

跨膜蛋白跨越膜磷脂雙層,這決定了跨膜域的強(qiáng)疏水性??缒さ鞍椎目缒び蚩赏耆虿糠植迦肽?nèi)??缒さ鞍追N類的多樣性決定了其功能特點(diǎn)的多樣性。它具有物質(zhì)轉(zhuǎn)運(yùn)、信號(hào)轉(zhuǎn)導(dǎo)能力。此外,在免疫應(yīng)答中,跨膜蛋白也發(fā)揮及其重要的作用。

人白細(xì)胞抗原(Human leukocyte antigen, HLA)是一類跨膜蛋白,是存在于機(jī)體細(xì)胞表面的一種蛋白或抗原,在機(jī)體免疫防御中起著至關(guān)重要的作用。HLA系統(tǒng)和MHC一起幫助身體的免疫系統(tǒng)區(qū)分自體物質(zhì)和外來(lái)物質(zhì)。

人白細(xì)胞抗原(HLA)在人群中的分布

HLA抗原在不同地區(qū)有不同的分布。以HLA-B*27為例,在美國(guó)哥倫比亞、加拿大沿海印第安人和加拿大海達(dá)人中,其陽(yáng)性率高達(dá)50%;在歐洲和美國(guó)的白種人中,B27陽(yáng)性率為6%到8%;日本和非洲黑人的B27陽(yáng)性率低于1%。

人白細(xì)胞抗原(HLA)應(yīng)用

HLA主要應(yīng)用于器官移植和輸血中供體和受體的匹配。

在器官移植中,供體和受體必須具有相同或匹配的HLA基因,以使供體組織不受受體免疫系統(tǒng)的攻擊或排斥。

在臨床輸血中,異體HLA免疫在輸血治療中引起各種問(wèn)題,如血小板輸注不應(yīng)性,發(fā)熱引起的非溶血性輸血反應(yīng),輸血相關(guān)的急性肺損傷,輸血相關(guān)的移植物抗宿主病等。因此,HLA檢測(cè)是非常必要的。

人白細(xì)胞抗原(HLA)和疾病

HLA基因與自身免疫疾病和癌癥有關(guān)。

HLA基因 相關(guān)疾病
HLA-B 強(qiáng)直性脊柱炎
HLA-DRB1 類風(fēng)濕性關(guān)節(jié)炎
HLA-A1、B8、DR17 自體免疫疾病
HLA-DR3 1型糖尿病

許多研究表明,HLA與HIV疾病有關(guān)。此外,HLA與妊娠期間復(fù)發(fā)性流產(chǎn),先兆子癇或新生兒溶血性疾病密切相關(guān)。HLA也是藥物敏感性的原因之一。

更多細(xì)節(jié)了解,如抗原處理途徑、人群分布、HLA多態(tài)性等,擴(kuò)展閱讀:Human Leukocyte Antigen: Distinguish between Friend and Foe.

在您的實(shí)驗(yàn)中,您可能需要HLA產(chǎn)品來(lái)輔助您的研究。CUSABIO可以幫助您。我們已經(jīng)建立了一個(gè)特殊的跨膜蛋白表達(dá)系統(tǒng)。目前已生產(chǎn)HLA相關(guān)產(chǎn)品如下:

Human leukocyte antigen (HLA) related proteins for your research

Target Product Name Code Expression System
HLA-B Recombinant Human HLA-B protein(HLA-B),partial CSB-RP150894h E.coli
HLA-C Recombinant Human HLA-C protein(HLA-C),partial CSB-RP150994h E.coli
HLA-DRA Recombinant Human HLA class II histocompatibility antigen, DR alpha chain(HLA-DRA),partial CSB-EP360793HU1 E.coli
HLA-DQA1 Recombinant Human HLA class II histocompatibility antigen, DQ alpha 1 chain(HLA-DQA1),partial CSB-RP148394h E.coli
HLA-DQA2 Recombinant Human HLA class II histocompatibility antigen, DQ alpha 2 chain(HLA-DQA2),partial CSB-RP148294h E.coli
HLA-DPA1 Recombinant Human HLA class II histocompatibility antigen, DP alpha 1 chain(HLA-DPA1),partial CSB-RP148194h E.coli
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G) CSB-MP010509HU Mammalian cell
HLA-DPB1 Recombinant Human HLA class II histocompatibility antigen, DP beta 1 chain(HLA-DPB1),partial CSB-RP148094h E.coli
HLA-DRB1 Recombinant Human HLA class II histocompatibility antigen, DRB1-1 beta chain(HLA-DRB1),partial CSB-EP361230HU E.coli
HLA-DRA Recombinant Human HLA class II histocompatibility antigen, DR alpha chain(HLA-DRA) CSB-EP360793HU E.coli
HLA-DQB1 Recombinant Human HLA class II histocompatibility antigen,DQ beta 1 chain(HLA-DQB1),partial CSB-EP355782HU E.coli
HLA-DMA Recombinant Human HLA-DMA protein(HLA-DMA),partial CSB-EP338892HU E.coli
HLA-DMB Recombinant Human HLA class II histocompatibility antigen, DM beta chain(HLA-DMB),partial CSB-EP333326HU E.coli
HLA-A Recombinant Human HLA class I histocompatibility antigen,A-1 alpha chain(HLA-A),partial CSB-EP328989HU E.coli
HLA-E Recombinant Human HLA class I histocompatibility antigen, alpha chain E(HLA-E) ,partial CSB-EP320269HU E.coli
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G) CSB-EP010509HUb0 E.coli
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G) CSB-EP010509HUa2 E.coli
HLA-DRA Recombinant Human HLA class II histocompatibility antigen, DR alpha chain(HLA-DRA) CSB-YP360793HU Yeast
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G),partial CSB-EP010509HU1 E.coli
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G) CSB-EP010509HU E.coli
HLA-E Recombinant Human HLA class I histocompatibility antigen, alpha chain E(HLA-E),partial CSB-BP320269HU Baculovirus
HLA-A Recombinant Human HLA class I histocompatibility antigen,A-1 alpha chain(HLA-A),partial CSB-EP328989HUb1 E.coli
HLA-A Recombinant Human HLA class I histocompatibility antigen, A alpha chain(HLA-A),partial CSB-BP328989HU Baculovirus
HLA-A Recombinant Human HLA class I histocompatibility antigen, A alpha chain(HLA-A),partial CSB-EP3499HU E.coli
HLA-DRB1 Recombinant Human HLA class II histocompatibility antigen, DRB1-3 chain(HLA-DRB1),partial CSB-EP365687HU E.coli
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G),partial CSB-EP010509HU1a6 E.coli
HLA-DRB5 Recombinant Human HLA class II histocompatibility antigen, DR beta 5 chain(HLA-DRB5),partial CSB-EP657235HU E.coli
HLA-G Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G) CSB-YP010509HU Yeast
HLA-DRB1 Recombinant Human HLA class II histocompatibility antigen, DRB1-12 beta chain(HLA-DRB1) CSB-EP846484HU E.coli

Human leukocyte antigen (HLA) related antibodies for your research

Target Product Name Code Species Reactivity Tested Applications
HLA-H HLA-H Antibody CSB-PA958630 Human ELISA, WB
HLA-H HLA-H Antibody CSB-PA009213 Human ELISA, WB
HLA-DQB1/HLA-DQB2 HLA-DQB1/HLA-DQB2 Antibody CSB-PA005915 Human ELISA, WB
HLA-DPA1 HLA-DPA1 Antibody CSB-PA14819A0Rb Human ELISA, WB, IF
HLA-DPA1 HLA-DPA1 Antibody CSB-PA177845 Human ELISA, WB
HLA-DOB HLA-DOB Antibody CSB-PA002935 Human ELISA, WB
HLA-DPA1 HLA-DPA1 Antibody CSB-PA009209 Human ELISA, WB
HLA-DOB HLA-DOB Antibody CSB-PA270928 Human ELISA, WB
HLA-A HLA-A Antibody CSB-PA906516 Human ELISA, WB
HLA-A HLA-A Antibody CSB-PA398166 Human ELISA, WB
HLA-DMB HLA-DMB Antibody CSB-PA561724 Human ELISA, IHC
HLA-DOA HLA-DOA Antibody CSB-PA002934 Human WB, IHC, ELISA
HLA-DOA HLA-DOA Antibody CSB-PA113671 Human ELISA, WB
HLA-DOA HLA-DOA Antibody CSB-PA356784ESR1HU Human ELISA, IHC

Human leukocyte antigen (HLA) related ELISA kits for your research

Target Product Name Code Sample Type Sensitivity
HLA-DRA Human HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA) ELISA kit CSB-EL010497HU serum, plasma, tissue homogenates 4.68 pg/mL
HLA-G Human leukocyte antigen G,HLA-G ELISA Kit CSB-E09401h serum, plasma, tissue homogenates 0.31 ng/mL
HLA-B27 Human leucocyte antigen B27,HLA-B27 ELISA Kit CSB-E08607h serum, urine, cerebrospinal fluid (CSF) 0.078 ng/mL
HLA-E Human major histocompatibility complex, class I, E (HLA-E) ELISA kit CSB-EL010507HU serum, plasma, cell culture supernates, tissue homogenates 7.81 pg/mL