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N Recombinant Monoclonal Antibody

  • 中文名稱：
    N重組抗體
  • 貨號：
    CSB-RA33255A0GMY
  • 規(guī)格：
    ￥2310
  • 圖片：
    • The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
      Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 6.892 ng/ml.
    • In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A0GMY) is clean, the detection limit can be as low as 446.4ng/ml (31.25ng/0.07ml), and the sensitivity is very good.
    • ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A0GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The recombinant N antibody was prepared by obtaining the antibody genes, cloning the genes into a plasma vector to construct vector clone, transfecting the vector clone into a mammalian cell line for transient expression, and purifying the antibody by affinity-chromatography. This recombinant N antibody has been verified to detect the N protein from Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) in the ELISA, GICA.

    SARS-CoV-2 N protein, a highly immunogenic viral protein, is encoded by the ninth ORF of the virus. It is involved in viral assembly, replication, and host immune response regulation. The N protein recognizes and binds to RNA, allowing for genome encapsidation and participating in the assembly of the virion through its interactions with the membrane protein M. It is an immunodominant antigen in host immune responses that can be used as a diagnostic antigen and immunogen. The N protein is more sensitive than the S protein in response to adaptive immunity, making it a better indicator of early disease.

  • Uniprot No.：
  • 別名：
    N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
  • 反應(yīng)種屬：
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原：
    Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)(CSB-EP3325GMY)
  • 免疫原種屬：
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Monoclonal mouse (varialbe region)/human (kappa/IgG1 constant)chimeric antibody
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    1A6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, GICA
  • 推薦稀釋比：
    Application Recommended Dilution
    ELISA 1:10000-1:50000
    GICA 1:500-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
  • 亞細胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Betacoronavirus nucleocapsid protein family