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  4. Recombinant Saccharomyces cerevisiae Alcohol-sensitive RING finger protein 1 (ASR1)

Recombinant Saccharomyces cerevisiae Alcohol-sensitive RING finger protein 1 (ASR1)

  • 中文名稱：
    Recombinant Alcohol-sensitive RING finger protein 1,Yeast
  • 貨號(hào)：
    CSB-YP312397
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Alcohol-sensitive RING finger protein 1,Yeast
  • 貨號(hào)：
    CSB-EP312397
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Alcohol-sensitive RING finger protein 1,Yeast
  • 貨號(hào)：
    CSB-EP312397-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Alcohol-sensitive RING finger protein 1,Yeast
  • 貨號(hào)：
    CSB-BP312397
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Alcohol-sensitive RING finger protein 1,Yeast
  • 貨號(hào)：
    CSB-MP312397
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ASR1
  • Uniprot No.：
  • 別名：
    ASR1; YPR093C; Alcohol-sensitive RING finger protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 表達(dá)區(qū)域：
    1-288
  • 氨基酸序列
    MEECPICLAD DQEGEQFGCL NVCGHKFHLN CIREWHKYSI NLKCPICRVE STHLEVGEGQ HALSINLKMG FMIKNAIDYV GAETTNERNE DDTGEQDQEI EFLSERLRGT LVMDTIKIIQ CSICGDTDVS RLSLYCQDCE AIYHETCLRG LACEVGDRNT WQECTDCRSN ALLELRMGAI SSQLASYDSR NSMIFAGELR DKHSVKTQQM YEQIRNAKHK IQMHVRRALD RYPLPLLRFK DAYKHVNKQV SRKLYRLSDN KYLPDQYDYD SLARTGVHTE LLIYCHDE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for tolerance to alcohol.
  • 基因功能參考文獻(xiàn)：
    1. A novel role for Asr1p in regulating cell cycle mediated by septin-dependent assembly in yeast.Asr1 involved in actin cytoskeleton influence the assembly of septin ring. PMID: 25646695
    2. a role of the Asr1p E3 ligase activity in coping with stress is proposed. PMID: 21726536
    3. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae. PMID: 21193817
    4. it was concluded that Asr1 is not required and nor important for tolerance to alcohol stress PMID: 16391921
    5. Asr1 is a RING finger ubiquitin-ligase that binds directly to RNA polymerase II via the carboxyl-terminal domain of the largest subunit of the enzyme. PMID: 19064926
    6. Deletion of ASR1 leads to sensitivity in growth on medium containing alcohol or detergent, consistent with a function of Asr1p in alcohol-related signaling. PMID: 15117954

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Accumulates in the nucleus specifically upon alcohol stress. Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YPR093C

    STRING: 4932.YPR093C