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  4. Recombinant Mouse TBC1 domain family member 4 (Tbc1d4), partial

Recombinant Mouse TBC1 domain family member 4 (Tbc1d4), partial

  • 中文名稱：
    小鼠Tbc1d4重組蛋白
  • 貨號(hào)：
    CSB-YP805989MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Tbc1d4重組蛋白
  • 貨號(hào)：
    CSB-EP805989MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Tbc1d4重組蛋白
  • 貨號(hào)：
    CSB-EP805989MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Tbc1d4重組蛋白
  • 貨號(hào)：
    CSB-BP805989MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Tbc1d4重組蛋白
  • 貨號(hào)：
    CSB-MP805989MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Tbc1d4; As160; Kiaa0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
  • 基因功能參考文獻(xiàn)：
    1. Fkbp5 knockouts are protected from high-fat diet-induced weight gain, show improved glucose tolerance and increased insulin signaling in skeletal muscle. We identify a novel association between FKBP51 and AS160, a substrate of AKT2 that is involved in glucose uptake. FKBP51 antagonism increases the phosphorylation of AS160. PMID: 29170369
    2. AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle. PMID: 27152871
    3. Findings demonstrate that the RabGAP activity of AS160 maintains GLUT4 protein levels in a cell/tissue-autonomous manner and its inactivation causes lysosomal degradation of GLUT4 and postprandial hyperglycemia and hyperinsulinemia. PMID: 27554475
    4. As160 knockout mice have GLUT4 expression and glucose uptake defects in skeletal muscle and adipose tissue. These defects combine in AS160 KO/Tbc1d1 KO mice, supporting nonredundant functions for AS160 and Tbc1d1. PMID: 26625902
    5. AS160 has a direct role in linking the trafficking of Na(+),K(+)-ATPase to the energy state of renal epithelial cells PMID: 25788531
    6. TBC1D4 is dispensable for the regulation of renal Na+ and water transport, but may play a role for GLUT4-mediated basolateral glucose uptake in distal tubules. PMID: 26336159
    7. The Thr649 phosphorylation of AS160/TBC1D4 by protein kinase B plays an important role in the heart electric conduction system through regulating the R-wave amplitude. PMID: 25923736
    8. AS160 forms a ternary complex with Fsp27 and Rab8a to positively regulate lipid droplets fusion. PMID: 25158853
    9. RAB10 and its GTPase-activating protein AS160 comprise the principal signaling module downstream of insulin receptor activation that regulates the accumulation of GLUT4 transport vesicles at the plasma membrane. PMID: 23804653
    10. Data show that the AS160-knockout mice exhibited insulin resistance in both muscle and liver in a euglycaemic clamp study. PMID: 23078342
    11. Constitutive targeting of AS160 to the plasma membrane increased the surface GLUT4 levels, and this was attributed to both enhanced AS160 phosphorylation and 14-3-3 binding and inhibition of AS160 GAP activity. PMID: 23045393
    12. TBC1D4 may play an important role for the control of renal ion and water handling and hence for the control of extracellular fluid homeostasis. PMID: 22466139
    13. In this study both PAS and Thr649 phosphorylation of immunoprecipitated AS160 were slightly decreased in various mouse skeletal muscle types upon in situ contraction or AICAR stimulation. PMID: 22318952
    14. AS160 and Rab8a regulate membrane recruitment of CD36. PMID: 22315395
    15. Leptin enhances the intracellular GLUT4 transport in skeletal muscle of ob/ob animals by reducing the expression and activity of the negative regulators of GLUT4 traffic TBC1D1 and TBC1D4. PMID: 22253718
    16. Loss of AS160 Akt substrate causes Glut4 protein to accumulate in compartments that are primed for fusion in basal adipocytes. PMID: 21613213
    17. These results suggest that FABP3 stimulates glucose uptake by facilitating AMPK-dependent AS160 phosphorylation in skeletal muscle. PMID: 21501347
    18. decreased expression of AS160 is likely to play a role in the translocation of aquaporin-2 to the plasma membrane PMID: 21511697
    19. Results provide genetic evidence that insulin-induced AS160-Thr649 phosphorylation and/or its binding to 14-3-3 play an important role in regulating whole-body glucose homeostasis. PMID: 21195350
    20. AS160, stabilizes ENaC in a regulated intracellular compartment under basal conditions and aldosterone/SGK1-dependent AS160 phosphorylation permits ENaC forward trafficking to the apical membrane to augment sodium absorption. PMID: 20410134
    21. S711 is a novel TBC1D4 phosphorylation site regulated by AMPK in skeletal muscle. PMID: 19923418
    22. AS160 was shown to be a substrate for Akt, and two sites of phosphorylation, both in RXRXXS/T motifs, were identified by mass spectrometry and mutation PMID: 11994271
    23. role for AS160 in the insulin regulated trafficking of GLUT4 PMID: 16154996
    24. AS160 is localized to GLUT4-containing vesicles via its interaction with IRAP where it inhibits the activity of Rab substrates in its vicinity, effectively tethering the vesicles intracellularly. PMID: 16762977
    25. AS160provides a mechanism linking AMP kinase signaling to glucose uptake. PMID: 16804075
    26. Insulin, contraction, and the AMP-activated protein kinase (AMPK) activator AICAR all increased AS160 phosphorylation in mouse skeletal muscle. PMID: 16804077
    27. insulin-dependent association of 14-3-3 with AS160 plays an important role in GLUT4 trafficking in adipocytes PMID: 16880201
    28. AS160 is a critical convergence factor for independent stimulators of skeletal muscle glucose uptake PMID: 16935857
    29. Rab10 is a target of AS160 and a positive regulator of GLUT4 trafficking to the cell surface upon insulin stimulation. PMID: 17403373
    30. AS160 calmodulin binding domain regulates contraction-induced glucose uptake in mouse muscle and calmodulin provides an additional means of modulating As160 protein. PMID: 17717281
    31. the FQQI motif within the amino terminus of GLUT4 is essential for GLUT4 endocytosis and AS160-dependent intracellular retention but not for the GGA-dependent sorting of GLUT4 into the insulin-responsive storage compartment PMID: 17761952
    32. demonstrate a proportional inhibition in both docking and fusion of GLUT4 storage vesicles by a dominant negative mutant of AS160 PMID: 18063571
    33. the Rab10 mutant considered to be constitutively active (Rab10 Q68L) is a substrate for the AS160 GAP domain and, hence, cannot be used to deduce rigorously the function of Rab10 in its GTP form PMID: 18076383
    34. AS160, previously recognized as a key player in insulin signaling in skeletal muscle and adipose tissue, is also a major effector of protein kinase B/Akt signaling in the beta-cell. PMID: 18276765
    35. GLUT4 vesicle recruitment and fusion are differentially regulated by Rac, AS160, and Rab8A in muscle cells. PMID: 18650435
    36. TBC1D4 acts to impede GLUT4 translocation by disarming a Rab protein found on GLUT4-containing-membranes. PMID: 18801932
    37. RUVBL2 plays an important role in insulin-stimulated GLUT4 translocation through its interaction with AS160 PMID: 19532121

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 組織特異性：
    Widely expressed, including in pancreatic beta cells.
  • 數(shù)據(jù)庫(kù)鏈接：