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  4. Recombinant Schizosaccharomyces pombe Cytoplasmic tRNA 2-thiolation protein 1 (ncs6)

Recombinant Schizosaccharomyces pombe Cytoplasmic tRNA 2-thiolation protein 1 (ncs6)

  • 中文名稱：
    龐貝裂殖酵母ncs6重組蛋白
  • 貨號：
    CSB-YP002426SXV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ncs6重組蛋白
  • 貨號：
    CSB-EP002426SXV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ncs6重組蛋白
  • 貨號：
    CSB-EP002426SXV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ncs6重組蛋白
  • 貨號：
    CSB-BP002426SXV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ncs6重組蛋白
  • 貨號：
    CSB-MP002426SXV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ncs6
  • Uniprot No.：
  • 別名：
    ncs6; ctu1; SPBC2G5.03Cytoplasmic tRNA 2-thiolation protein 1; EC 2.7.7.-; Cytoplasmic tRNA adenylyltransferase 1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-335
  • 氨基酸序列
    MSNKLCQLCN ERRPALVRPK TGQKICKECF YYVFETEIHN VIIENKLFVR GERVGIGASG GKDSTVLAYV MKLLNERYDY GLELYLISVD EGIRGYRDDS LDTVKRNQQQ YGLPMKIVSY ADLYDGWTMD NVVARIGTKN NCTYCGVFRR QALDRAALSL DIHHLVTGHN ADDIAETILM NLLRGDVARL PRSTEITTQS DSSPTKRSKP FKYSYEKEIV LYAHYKKLDY FSTECTYSPE AFRGTARAMI KQLENIRPSS ILDIIYSGES MQLASSVQEQ LPQQTTCERC GFISSNRICK ACMLLEGLNK GITGLGLGSD RKTKKLQSQI PACAE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    TtcA family, CTU1/NCS6/ATPBD3 subfamily
  • 數(shù)據(jù)庫鏈接：