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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfurihydrogenibium sp. NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP458921FPP1
CSB-EP458921FPP1
CSB-BP458921FPP1
CSB-MP458921FPP1
CSB-EP458921FPP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP458922FPP
CSB-EP458922FPP
CSB-BP458922FPP
CSB-MP458922FPP
CSB-EP458922FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Isoleucine--tRNA ligase (ileS), partial CSB-YP458923FPP
CSB-EP458923FPP
CSB-BP458923FPP
CSB-MP458923FPP
CSB-EP458923FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Acireductone dioxygenase (mtnD) CSB-YP458924FPP
CSB-EP458924FPP
CSB-BP458924FPP
CSB-MP458924FPP
CSB-EP458924FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cysteine--tRNA ligase (cysS) CSB-YP458925ENB
CSB-EP458925ENB
CSB-BP458925ENB
CSB-MP458925ENB
CSB-EP458925ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein TolB (tolB) CSB-YP458926ENB
CSB-EP458926ENB
CSB-BP458926ENB
CSB-MP458926ENB
CSB-EP458926ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Biotin synthase (bioB) CSB-YP458927ENB
CSB-EP458927ENB
CSB-BP458927ENB
CSB-MP458927ENB
CSB-EP458927ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Serine--tRNA ligase (serS) CSB-YP458928ENB
CSB-EP458928ENB
CSB-BP458928ENB
CSB-MP458928ENB
CSB-EP458928ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA replication and repair protein recF (recF) CSB-YP458929ENB
CSB-EP458929ENB
CSB-BP458929ENB
CSB-MP458929ENB
CSB-EP458929ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP458930ENB
CSB-EP458930ENB
CSB-BP458930ENB
CSB-MP458930ENB
CSB-EP458930ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0133 protein ETA_24730 (ETA_24730) CSB-YP458931ENB
CSB-EP458931ENB
CSB-BP458931ENB
CSB-MP458931ENB
CSB-EP458931ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP458932ENB
CSB-EP458932ENB
CSB-BP458932ENB
CSB-MP458932ENB
CSB-EP458932ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP458933ENB
CSB-EP458933ENB
CSB-BP458933ENB
CSB-MP458933ENB
CSB-EP458933ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP458934ENB
CSB-EP458934ENB
CSB-BP458934ENB
CSB-MP458934ENB
CSB-EP458934ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Serine/threonine transporter sstT (sstT), partial CSB-YP458935ENB
CSB-EP458935ENB
CSB-BP458935ENB
CSB-MP458935ENB
CSB-EP458935ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP458936ENB
CSB-EP458936ENB
CSB-BP458936ENB
CSB-MP458936ENB
CSB-EP458936ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable manganese transport protein MntH (mntH), partial CSB-YP458937ENB
CSB-EP458937ENB
CSB-BP458937ENB
CSB-MP458937ENB
CSB-EP458937ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP458938ENB
CSB-EP458938ENB
CSB-BP458938ENB
CSB-MP458938ENB
CSB-EP458938ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA ligase (ligA), partial CSB-YP458939ENB
CSB-EP458939ENB
CSB-BP458939ENB
CSB-MP458939ENB
CSB-EP458939ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP458940ENB
CSB-EP458940ENB
CSB-BP458940ENB
CSB-MP458940ENB
CSB-EP458940ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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