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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrenophora tritici-repentis Molybdopterin synthase sulfur carrier subunit (mocs2) CSB-YP458982FHT
CSB-EP458982FHT
CSB-BP458982FHT
CSB-MP458982FHT
CSB-EP458982FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Probable Xaa-Pro aminopeptidase pepP (pepP) CSB-YP458983FHT
CSB-EP458983FHT
CSB-BP458983FHT
CSB-MP458983FHT
CSB-EP458983FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oedogonium cardiacum ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP458984ODX1
CSB-EP458984ODX1
CSB-BP458984ODX1
CSB-MP458984ODX1
CSB-EP458984ODX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oedogonium cardiacum 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP458985ODX
CSB-EP458985ODX
CSB-BP458985ODX
CSB-MP458985ODX
CSB-EP458985ODX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo Photosystem II D2 protein (psbD1), partial CSB-YP458986HWH1
CSB-EP458986HWH1
CSB-BP458986HWH1
CSB-MP458986HWH1
CSB-EP458986HWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP458987HWH1
CSB-EP458987HWH1
CSB-BP458987HWH1
CSB-MP458987HWH1
CSB-EP458987HWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo Photosystem II reaction center protein ycf12 (ycf12) CSB-YP458988HWG
CSB-EP458988HWG
CSB-BP458988HWG
CSB-MP458988HWG
CSB-EP458988HWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP458989FEC1
CSB-EP458989FEC1
CSB-BP458989FEC1
CSB-MP458989FEC1
CSB-EP458989FEC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP458990FEC1
CSB-EP458990FEC1
CSB-BP458990FEC1
CSB-MP458990FEC1
CSB-EP458990FEC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP458991FEC
CSB-EP458991FEC
CSB-BP458991FEC
CSB-MP458991FEC
CSB-EP458991FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP458992FEC
CSB-EP458992FEC
CSB-BP458992FEC
CSB-MP458992FEC
CSB-EP458992FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP458993WBL
CSB-EP458993WBL
CSB-BP458993WBL
CSB-MP458993WBL
CSB-EP458993WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP458994WBL
CSB-EP458994WBL
CSB-BP458994WBL
CSB-MP458994WBL
CSB-EP458994WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP458995WBL1
CSB-EP458995WBL1
CSB-BP458995WBL1
CSB-MP458995WBL1
CSB-EP458995WBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP458996WBL
CSB-EP458996WBL
CSB-BP458996WBL
CSB-MP458996WBL
CSB-EP458996WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP458997WBL
CSB-EP458997WBL
CSB-BP458997WBL
CSB-MP458997WBL
CSB-EP458997WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti Huwentoxin-11g3 CSB-YP458998HUL
CSB-EP458998HUL
CSB-BP458998HUL
CSB-MP458998HUL
CSB-EP458998HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphatidylcholine:ceramide cholinephosphotransferase 4 (SLS4), partial CSB-YP458999TQV1
CSB-EP458999TQV1
CSB-BP458999TQV1
CSB-MP458999TQV1
CSB-EP458999TQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Melanoplus sanguinipes Serine protease inhibitor 1 CSB-YP459000MQU
CSB-EP459000MQU
CSB-BP459000MQU
CSB-MP459000MQU
CSB-EP459000MQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Glycine and serine-rich protein 1 CSB-YP459001LQP
CSB-EP459001LQP
CSB-BP459001LQP
CSB-MP459001LQP
CSB-EP459001LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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