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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phymatum 10 kDa chaperonin (groS) CSB-YP460321BXT
CSB-EP460321BXT
CSB-BP460321BXT
CSB-MP460321BXT
CSB-EP460321BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Elongation factor P (efp) CSB-YP460322BXT
CSB-EP460322BXT
CSB-BP460322BXT
CSB-MP460322BXT
CSB-EP460322BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L9 (rplI) CSB-YP460323BXT
CSB-EP460323BXT
CSB-BP460323BXT
CSB-MP460323BXT
CSB-EP460323BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Transcriptional repressor NrdR (nrdR) CSB-YP460324BXT
CSB-EP460324BXT
CSB-BP460324BXT
CSB-MP460324BXT
CSB-EP460324BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP460325BXT
CSB-EP460325BXT
CSB-BP460325BXT
CSB-MP460325BXT
CSB-EP460325BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Probable intracellular septation protein A (Bphy_1025), partial CSB-YP460326BXT1
CSB-EP460326BXT1
CSB-BP460326BXT1
CSB-MP460326BXT1
CSB-EP460326BXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Gamma-glutamyl phosphate reductase (proA) CSB-YP460327BXT
CSB-EP460327BXT
CSB-BP460327BXT
CSB-MP460327BXT
CSB-EP460327BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP460328BXT
CSB-EP460328BXT
CSB-BP460328BXT
CSB-MP460328BXT
CSB-EP460328BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP460329BXT
CSB-EP460329BXT
CSB-BP460329BXT
CSB-MP460329BXT
CSB-EP460329BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Sec-independent protein translocase protein TatA (tatA), partial CSB-YP460330BXT
CSB-EP460330BXT
CSB-BP460330BXT
CSB-MP460330BXT
CSB-EP460330BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L15 (rplO) CSB-YP460331BXT
CSB-EP460331BXT
CSB-BP460331BXT
CSB-MP460331BXT
CSB-EP460331BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L24 (rplX) CSB-YP460332BXT
CSB-EP460332BXT
CSB-BP460332BXT
CSB-MP460332BXT
CSB-EP460332BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L16 (rplP) CSB-YP460333BXT
CSB-EP460333BXT
CSB-BP460333BXT
CSB-MP460333BXT
CSB-EP460333BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L2 (rplB) CSB-YP460334BXT
CSB-EP460334BXT
CSB-BP460334BXT
CSB-MP460334BXT
CSB-EP460334BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP460335BXT
CSB-EP460335BXT
CSB-BP460335BXT
CSB-MP460335BXT
CSB-EP460335BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP460336BXT
CSB-EP460336BXT
CSB-BP460336BXT
CSB-MP460336BXT
CSB-EP460336BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Adenylosuccinate synthetase (purA) CSB-YP460337BXT
CSB-EP460337BXT
CSB-BP460337BXT
CSB-MP460337BXT
CSB-EP460337BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L20 (rplT) CSB-YP460338BXT
CSB-EP460338BXT
CSB-BP460338BXT
CSB-MP460338BXT
CSB-EP460338BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP460339BXT
CSB-EP460339BXT
CSB-BP460339BXT
CSB-MP460339BXT
CSB-EP460339BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 7-cyano-7-deazaguanine synthase (queC) CSB-YP460340BXT
CSB-EP460340BXT
CSB-BP460340BXT
CSB-MP460340BXT
CSB-EP460340BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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