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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila persimilis Protein crossbronx (cbx) CSB-YP459602DMB
CSB-EP459602DMB
CSB-BP459602DMB
CSB-MP459602DMB
CSB-EP459602DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Eukaryotic translation initiation factor 3 subunit F-2 (eIF3-S5-2) CSB-YP459603DMB
CSB-EP459603DMB
CSB-BP459603DMB
CSB-MP459603DMB
CSB-EP459603DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Calcium channel flower (flower), partial CSB-YP459604DMG1
CSB-EP459604DMG1
CSB-BP459604DMG1
CSB-MP459604DMG1
CSB-EP459604DMG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein cueball (cue), partial CSB-YP459605DMG
CSB-EP459605DMG
CSB-BP459605DMG
CSB-MP459605DMG
CSB-EP459605DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Adenylate kinase 2, mitochondrial (Adk2-1) CSB-YP459606DMG
CSB-EP459606DMG
CSB-BP459606DMG
CSB-MP459606DMG
CSB-EP459606DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Eukaryotic translation initiation factor 3 subunit F-1 (eIF3-S5-1) CSB-YP459607DMG
CSB-EP459607DMG
CSB-BP459607DMG
CSB-MP459607DMG
CSB-EP459607DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Cytosolic Fe-S cluster assembly factor NUBP1 homolog (GM17199) CSB-YP459608DMG
CSB-EP459608DMG
CSB-BP459608DMG
CSB-MP459608DMG
CSB-EP459608DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein Turandot B (TotB) CSB-YP459609DMG
CSB-EP459609DMG
CSB-BP459609DMG
CSB-MP459609DMG
CSB-EP459609DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Enhancer of yellow 2 transcription factor (e (y)2) CSB-YP459610DMG
CSB-EP459610DMG
CSB-BP459610DMG
CSB-MP459610DMG
CSB-EP459610DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Probable cytosolic Fe-S cluster assembly factor GM20417 (GM20417) CSB-YP459611DMG
CSB-EP459611DMG
CSB-BP459611DMG
CSB-MP459611DMG
CSB-EP459611DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Queuine tRNA-ribosyltransferase subunit QTRTD1 homolog (GH16210) CSB-YP459612DLL
CSB-EP459612DLL
CSB-BP459612DLL
CSB-MP459612DLL
CSB-EP459612DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Cytosolic Fe-S cluster assembly factor NUBP2 homolog (GH14587) CSB-YP459613DLL
CSB-EP459613DLL
CSB-BP459613DLL
CSB-MP459613DLL
CSB-EP459613DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein wntless (wls), partial CSB-YP459614DLL1
CSB-EP459614DLL1
CSB-BP459614DLL1
CSB-MP459614DLL1
CSB-EP459614DLL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Eukaryotic translation initiation factor 3 subunit G-1 (eIF3-S4-1) CSB-YP459615DLL
CSB-EP459615DLL
CSB-BP459615DLL
CSB-MP459615DLL
CSB-EP459615DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Adenylate kinase 2, mitochondrial (Adk2) CSB-YP459616DLL
CSB-EP459616DLL
CSB-BP459616DLL
CSB-MP459616DLL
CSB-EP459616DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 homolog (GH21310), partial CSB-YP459617DLL
CSB-EP459617DLL
CSB-BP459617DLL
CSB-MP459617DLL
CSB-EP459617DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein hook (hk), partial CSB-YP459618DLL
CSB-EP459618DLL
CSB-BP459618DLL
CSB-MP459618DLL
CSB-EP459618DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Glutamyl-tRNA (Gln) amidotransferase subunit C, mitochondrial, partial CSB-YP459619DLL
CSB-EP459619DLL
CSB-BP459619DLL
CSB-MP459619DLL
CSB-EP459619DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi tRNA (guanine-N (7)-)-methyltransferase CSB-YP459620DLL
CSB-EP459620DLL
CSB-BP459620DLL
CSB-MP459620DLL
CSB-EP459620DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Ribosome biogenesis protein WDR12 homolog (GH13339) CSB-YP459621DLL
CSB-EP459621DLL
CSB-BP459621DLL
CSB-MP459621DLL
CSB-EP459621DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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