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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP460201XBN
CSB-EP460201XBN
CSB-BP460201XBN
CSB-MP460201XBN
CSB-EP460201XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipid-A-disaccharide synthase (lpxB) CSB-YP460202XBN
CSB-EP460202XBN
CSB-BP460202XBN
CSB-MP460202XBN
CSB-EP460202XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP460203XBN
CSB-EP460203XBN
CSB-BP460203XBN
CSB-MP460203XBN
CSB-EP460203XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP460204XBN
CSB-EP460204XBN
CSB-BP460204XBN
CSB-MP460204XBN
CSB-EP460204XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S19 (rpsS) CSB-YP460205XBN
CSB-EP460205XBN
CSB-BP460205XBN
CSB-MP460205XBN
CSB-EP460205XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S14 (rpsN) CSB-YP460206XBN
CSB-EP460206XBN
CSB-BP460206XBN
CSB-MP460206XBN
CSB-EP460206XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP460207XBN
CSB-EP460207XBN
CSB-BP460207XBN
CSB-MP460207XBN
CSB-EP460207XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 5'-nucleotidase surE (surE) CSB-YP460208XBN
CSB-EP460208XBN
CSB-BP460208XBN
CSB-MP460208XBN
CSB-EP460208XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP460209XBN
CSB-EP460209XBN
CSB-BP460209XBN
CSB-MP460209XBN
CSB-EP460209XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Siroheme synthase (cysG) CSB-YP460210XBN
CSB-EP460210XBN
CSB-BP460210XBN
CSB-MP460210XBN
CSB-EP460210XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA (guanine-N (7)-)-methyltransferase CSB-YP460211XBN
CSB-EP460211XBN
CSB-BP460211XBN
CSB-MP460211XBN
CSB-EP460211XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Shikimate kinase (aroK) CSB-YP460212XBN
CSB-EP460212XBN
CSB-BP460212XBN
CSB-MP460212XBN
CSB-EP460212XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Tryptophan synthase alpha chain (trpA) CSB-YP460213XBN
CSB-EP460213XBN
CSB-BP460213XBN
CSB-MP460213XBN
CSB-EP460213XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L35 (rpmI) CSB-YP460214XBN
CSB-EP460214XBN
CSB-BP460214XBN
CSB-MP460214XBN
CSB-EP460214XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP460215XBN
CSB-EP460215XBN
CSB-BP460215XBN
CSB-MP460215XBN
CSB-EP460215XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP460216XBN
CSB-EP460216XBN
CSB-BP460216XBN
CSB-MP460216XBN
CSB-EP460216XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glutamyl-tRNA reductase (hemA) CSB-YP460217XBN
CSB-EP460217XBN
CSB-BP460217XBN
CSB-MP460217XBN
CSB-EP460217XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L13 (rplM) CSB-YP460218XBN
CSB-EP460218XBN
CSB-BP460218XBN
CSB-MP460218XBN
CSB-EP460218XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Protein hfq (hfq) CSB-YP460219BSP
CSB-EP460219BSP
CSB-BP460219BSP
CSB-MP460219BSP
CSB-EP460219BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica DNA repair protein recO (recO) CSB-YP460220BSP
CSB-EP460220BSP
CSB-BP460220BSP
CSB-MP460220BSP
CSB-EP460220BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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