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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phenylobacterium zucineum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP459681EUP
CSB-EP459681EUP
CSB-BP459681EUP
CSB-MP459681EUP
CSB-EP459681EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum DNA mismatch repair protein mutL (mutL), partial CSB-YP459682EUP
CSB-EP459682EUP
CSB-BP459682EUP
CSB-MP459682EUP
CSB-EP459682EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Probable transaldolase (tal) CSB-YP459683EUP
CSB-EP459683EUP
CSB-BP459683EUP
CSB-MP459683EUP
CSB-EP459683EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Biotin synthase (bioB) CSB-YP459684EUP
CSB-EP459684EUP
CSB-BP459684EUP
CSB-MP459684EUP
CSB-EP459684EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP459685EUP
CSB-EP459685EUP
CSB-BP459685EUP
CSB-MP459685EUP
CSB-EP459685EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP459686EUP
CSB-EP459686EUP
CSB-BP459686EUP
CSB-MP459686EUP
CSB-EP459686EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Glutamate racemase (murI) CSB-YP459687EUP
CSB-EP459687EUP
CSB-BP459687EUP
CSB-MP459687EUP
CSB-EP459687EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Tryptophan synthase beta chain (trpB) CSB-YP459688EUP
CSB-EP459688EUP
CSB-BP459688EUP
CSB-MP459688EUP
CSB-EP459688EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP459689EUP
CSB-EP459689EUP
CSB-BP459689EUP
CSB-MP459689EUP
CSB-EP459689EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Aspartate--tRNA ligase (aspS), partial CSB-YP459690EUP
CSB-EP459690EUP
CSB-BP459690EUP
CSB-MP459690EUP
CSB-EP459690EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Orotate phosphoribosyltransferase (pyrE) CSB-YP459691EUP
CSB-EP459691EUP
CSB-BP459691EUP
CSB-MP459691EUP
CSB-EP459691EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Glutamate 5-kinase (proB) CSB-YP459692EUP
CSB-EP459692EUP
CSB-BP459692EUP
CSB-MP459692EUP
CSB-EP459692EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Serine--tRNA ligase (serS) CSB-YP459693EUP
CSB-EP459693EUP
CSB-BP459693EUP
CSB-MP459693EUP
CSB-EP459693EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP459694EUP
CSB-EP459694EUP
CSB-BP459694EUP
CSB-MP459694EUP
CSB-EP459694EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UPF0262 protein PHZ_c2197 (PHZ_c2197) CSB-YP459695EUP
CSB-EP459695EUP
CSB-BP459695EUP
CSB-MP459695EUP
CSB-EP459695EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP459696EUP
CSB-EP459696EUP
CSB-BP459696EUP
CSB-MP459696EUP
CSB-EP459696EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP459697EUP
CSB-EP459697EUP
CSB-BP459697EUP
CSB-MP459697EUP
CSB-EP459697EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Chorismate synthase (aroC) CSB-YP459698EUP
CSB-EP459698EUP
CSB-BP459698EUP
CSB-MP459698EUP
CSB-EP459698EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP459699NGB
CSB-EP459699NGB
CSB-BP459699NGB
CSB-MP459699NGB
CSB-EP459699NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L32 (rpmF) CSB-YP459700NGB
CSB-EP459700NGB
CSB-BP459700NGB
CSB-MP459700NGB
CSB-EP459700NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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